Overexpression of BnKCS1-1, BnKCS1-2, and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus
Overexpression of BnKCS1-1, BnKCS1-2, and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus
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BnKCS1-1、BnKCS1-2 和 BnCER1-2 的过表达可促进甘蓝型油菜角质层蜡的产生并提高耐旱性
DOI：
10.1016/j.cj.2019.04.006
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发表时间：
2020-02
期刊：
影响因子：
6.6
通讯作者：
Ni Yu
中科院分区：
文献类型：
--
作者：
Wang Yanmei;Jin Shurong;Xu Yi;Li Shuai;Zhang Shuangjuan;Yuan Zheng;Li Jiana;Ni Yu
植物中较高量的表皮蜡与改善的植物胁迫耐受性和增加的工业用途潜力有关。在本研究中，拟南芥中的KCS 1和CER 1的直向同源物，命名为BnKCS 1 -1，BnKCS 1 -2和BnCER 1 -2，从甘蓝型油菜中分离。BnKCS 1 - 1和BnKCS 1 - 2在B.阿坝和干旱处理均能诱导BnCER 1 - 2基因的转录，而干旱处理仅能诱导BnCER 1 - 2基因的转录。这三个基因的转录本显着下降，当植物与茉莉酸甲酯（MeJA）处理或受到冷胁迫。在CaMV 35 S启动子控制下，BnKCS 1 -1、BnKCS 1 -2和BnCER 1 - 2的过表达导致转基因B细胞表皮蜡的显著增加。BnKCS 1 - 1和BnKCS 1 - 2的过表达导致了与非转化植株相似的差异，具有显著更高水平的醛（C29和C30）、烷烃（C28、C29和C31）和仲醇（C28和C29），以及显著更低水平的C29酮。BnCER 1 - 2的过表达导致烷烃（C27、C28、C29和C31）的增加，仲醇（C28和C29）的减少，而其他蜡组分的变化不明显。扫描电镜显示BnKCS 1 -1、BnKCS 1 -2和BnCER 1 - 2在B. napus导致在叶表面上比在非转化植物中观察到的更高密度的蜡晶体。与非转化植物相比，转基因植物表现出降低的水分损失率和增加的耐旱性。这些结果表明，BnKCS 1 -1、BnKCS 1 -2和BnCER 1 - 2基因产物可以修饰B.油菜。利用转基因技术改变植物表皮蜡质是植物耐旱性遗传改良的新策略，也为B.油菜是一种表面蜡质作物。
Higher amounts of cuticular wax in plants have been associated with improved plant stress tolerance and increased potential for industrial use. In this study, orthologs ofKCS1andCER1inArabidopsis, designatedBnKCS1-1,BnKCS1-2, andBnCER1-2, were isolated fromBrassica napus. Transcription ofBnKCS1-1andBnKCS1-2inB. napuswere induced by abscisic acid (ABA) and drought treatment, while transcription ofBnCER1-2was induced only by drought treatment. All three gene transcripts decreased significantly when plants were treated with methyl jasmonate (MeJA) or subjected to cold stress. Overexpression ofBnKCS1-1,BnKCS1-2, andBnCER1-2under the control of the CaMV35S promoter led to a significant increase in cuticular wax on transgenicB. napusleaves.BnKCS1-1andBnKCS1-2overexpression led to similar differences from non-transformed plants, with significantly higher levels of aldehydes (C29and C30), alkanes (C28, C29, and C31) and secondary alcohols (C28and C29), and a significantly lower level of C29ketone. Overexpression ofBnCER1-2led to an increase in alkanes (C27, C28, C29, and C31), a decrease in secondary alcohols (C28and C29), and insignificant changes in other wax components. Scanning electron microscopy revealed that overexpression ofBnKCS1-1,BnKCS1-2, andBnCER1-2inB. napusresulted in a higher density of wax crystals on the leaf surface than observed in non-transformed plants. Transgenic plants showed a reduced rate of water loss and increased drought tolerance compared to non-transformed plants. These results suggest thatBnKCS1-1,BnKCS1-2, andBnCER1-2gene products can modify the cuticular wax ofB. napus. Changing cuticular waxes using transgenic approaches is a new strategy for genetic improvement of plant drought tolerance and provides an opportunity for development ofB. napusas a surface-wax crop.
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影响因子：
11.6
作者：
Fiebig, A;Mayfield, JA;Preuss, D
通讯作者：
Preuss, D
DOI：
10.1073/pnas.0404600101
发表时间：
2004-08
影响因子：
11.1
作者：
S. Trenkamp;W. Martin;K. Tietjen
通讯作者：
S. Trenkamp;W. Martin;K. Tietjen
影响因子：
6.2
作者：
Cardoza, V;Stewart, CN
通讯作者：
Stewart, CN
植物中 KCS 基因家族的进化：基因复制、亚/新功能化和冗余的历史
影响因子：
3.1
作者：
Guo, Hai-Song;Zhang, Yan-Mei;Xue, Jia-Yu
通讯作者：
Xue, Jia-Yu
影响因子：
7.4
作者：
Hooker, TS;Millar, AA;Kunst, L
通讯作者：
Kunst, L