New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.
New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.
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DOI：
10.1371/journal.pone.0093394
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jiang YJ
中科院分区：
文献类型：
--
作者：
Tseng LC;Zhang C;Cheng CM;Xu H;Hsu CH;Jiang YJ
Notch信号通路通过局部细胞相互作用在广泛的发育过程中定义了一种进化保守的机制来决定细胞的命运。心灵炸弹(Mind Bomb，MIB)编码一个E3泛素连接酶，通过Delta泛素化和内化参与Notch的激活。为了进一步分析Mib的功能，我们进行了两种不同策略的斑马鱼Mib/MIB2结合蛋白的酵母双杂交筛选。发现了81个可能感兴趣的蛋白质，分为6类：泛素蛋白酶体途径、细胞骨架、运输、复制/转录/翻译因子、细胞信号转导和其他。经免疫共沉淀(Co-IP)证实，Mib与四种被测蛋白相互作用：泛素特异性蛋白水解酶1(USP1)、泛素特异性蛋白水解酶9(USP9)、肿瘤坏死因子受体相关因子结合域(TRAF)/锌指、含1(Zranb1)和低氧诱导因子1(Zranb1)的Ran结合域、α亚单位抑制物(HIF1AN)/抑制HIF-1(FIH-1)。Usp1、Usp9、Traid和FIH-1也与斑马鱼MIB2结合，MIB2是具有相似结构域和功能的MIB同源物。MIb和MIb2均能泛素化TRABID和FIH1，提示MIb和MIb2对TRABID和FIH1有潜在的调控作用，并且Notch信号可能参与了低氧调控的分化、肿瘤发生和NF-κB通路。最后，对已证实的Mib/MIB2相互作用蛋白的功能进行了整理、总结和假设，它描绘了一个超越Notch信号的调控网络。
Notch signaling pathway defines an evolutionarily conserved mechanism in cell-fate determination in a broad spectrum of developmental processes through local cell interactions. mind bomb (mib) encodes an E3 ubiquitin ligase that is involved in Notch activation through Delta ubiquitylation and internalization. To further dissect the function of Mib, two yeast two-hybrid screens for zebrafish Mib/Mib2-binding proteins with different strategies have been performed. 81 putative interesting proteins were discovered and classified into six groups: ubiquitin proteasome pathway, cytoskeleton, trafficking, replication/transcription/translation factors, cell signaling and others. Confirmed by coimmunoprecipitation (Co-IP), Mib interacted with four tested proteins: ubiquitin specific protease 1 (Usp1), ubiquitin specific protease 9 (Usp9), tumor-necrosis-factor-receptor-associated factor (TRAF)-binding domain (Trabid)/zinc finger, RAN-binding domain containing 1 (Zranb1) and hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an)/factor inhibiting HIF 1 (Fih-1). Usp1, Usp9, Trabid and Fih-1 also bound to zebrafish Mib2, a Mib homolog with similar structural domains and functions. Both Mib and Mib2 can ubiquitylate Trabid and Fih-1, indicating a potential regulating role of Mib and Mib2 on Trabid and Fih-1 and, furthermore, the possible involvement of Notch signaling in hypoxia-regulated differentiation, tumorigenesis and NF-κB pathway. Finally, functions of confirmed Mib/Mib2-interacting proteins are collated, summarized and hypothesized, which depicts a regulating network beyond Notch signaling.
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DOI：
10.1083/jcb.201107021
发表时间：
2011-09-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Berndt JD;Aoyagi A;Yang P;Anastas JN;Tang L;Moon RT
通讯作者：
Moon RT
DOI：
10.1073/pnas.1203605109
发表时间：
2012-05-08
影响因子：
11.1
作者：
Horn, Signe;Kobberup, Sune;Jensen, Jan N.
通讯作者：
Jensen, Jan N.
影响因子：
4.8
作者：
Coleman, Mathew L.;McDonough, Michael A.;Schofield, Christopher J.
通讯作者：
Schofield, Christopher J.
影响因子：
64.8
作者：
Jiang, YJ;Aerne, BL;Lewis, J
通讯作者：
Lewis, J
影响因子：
11.8
作者：
Itoh, M;Kim, CH;Chitnis, AB
通讯作者：
Chitnis, AB