A micromachined force sensing apparatus and method for human engineered cardiac tissue and induced pluripotent stem cell characterization.
A micromachined force sensing apparatus and method for human engineered cardiac tissue and induced pluripotent stem cell characterization.
复制标题
用于人体工程心脏组织和诱导多能干细胞表征的微机械力传感装置和方法。
DOI：
10.1016/j.sna.2021.112874
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Gaitas,Angelo
中科院分区：
文献类型：
--
作者：
Turnbull,IreneC;Zhu,Weibin;Stillitano,Francesca;Chien,Chen-Chi;Gaitas,Angelo
诱导多能干细胞来源的心肌细胞(IPSC-CMS)在细胞治疗、药物评价以及了解心脏疾病的病理生理学和遗传学基础方面具有巨大的潜力。收缩力量是心脏功能最重要的特征之一，是健康和疾病状态的预测因子。悬臂技术，如原子力显微镜，测量单个细胞的垂直力，而设计成更接近物理心脏功能的系统，如由末端柱固定的工程心脏组织，测量轴向力。一个重要的问题是，这两种力的测量是如何相互关联的？通过建立轴向力和垂直力之间的关联，我们将离能够使用单细胞IPSC-CMS作为模型又近了一步。研制了一种用于工程组织力收缩测量的新型微机械传感器。利用这种新型传感器，实验建立了轴向力和垂直力之间的关系。这一发现支持使用垂直力测量作为组织轴向力测量的替代方法。
Induced pluripotent stem cell derived-cardiomyocytes (iPSC-CMs) have great potential for cell therapy, drug assessment, and for understanding the pathophysiology and genetic underpinnings of cardiac diseases. Contraction forces are one of the most important characteristics of cardiac function and are predictors of healthy and diseased states. Cantilever techniques, such as atomic force microscopy, measure the vertical force of a single cell, while systems designed to more closely resemble the physical heart function, such as engineered cardiac tissue held by end-posts, measure the axial force. One important question is how do these two force measurements correlate? By establishing a correlation of the axial and vertical force, we will be one step closer in being able to use single cell iPSC-CMs as models. A novel micromachined sensor for measuring force contractions of engineered tissue has been developed. Using this novel sensor, a correlation between axial and vertical forces is experimentally established. This finding supports the use of vertical force measurements as an alternative to tissue axial force measurements.
登录
查看更多内容
影响因子：
1.6
作者：
Galende, Elisa;Karakikes, Ioannis;Polgar, Katalin
通讯作者：
Polgar, Katalin
影响因子：
2.4
作者：
Mathur, AB;Collinsworth, AM;Truskey, GA
通讯作者：
Truskey, GA
使用原子力显微镜以纳米级力调节微图案心肌细胞的收缩性
影响因子：
--
作者：
N. Nagarajan;Varun Vyas;B. Huey;P. Zorlutuna
通讯作者：
P. Zorlutuna
DOI：
10.1063/1.4915500
发表时间：
2015-03
期刊：
The Review of scientific instruments
影响因子：
--
作者：
A. Gaitas;R. Malhotra;Tao Li;T. Herron;J. Jalife
通讯作者：
A. Gaitas;R. Malhotra;Tao Li;T. Herron;J. Jalife
Suzuki S：“从慢速起搏到心动过速起搏的耦合间隔决定了持续的交替模式”Am J Physiol Heart Circ Physiol。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--