Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
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大白菜（Brassica rapa L. ssp. pekinensis）雄性不育AB系花药特异性启动子的鉴定
DOI：
10.1007/s13205-022-03160-z
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发表时间：
2022-03
期刊：
影响因子：
2.8
通讯作者：
Hui Feng
中科院分区：
文献类型：
--
作者：
Ying Zhao;Ying Sun;Shengnan Huang;Zhiyong Liu;Hui Feng
水稻雄性不育基因启动子的研究对研究水稻雄性不育具有重要意义。本研究利用RNA-seq技术从大白菜核不育AB系中鉴定出在不育株和可育株间差异表达的基因BraA 08 g 014780. 3C。qRT-PCR结果表明，BraA08g014780.3C主要在可育植株的花芽发育早期表达，且优先在花药中表达。对BraA08g014780.3C启动子进行克隆和分析，顺式作用元件分析表明，BraA08g014780.3C启动子含有花粉特异表达元件POLLEN 1 LELAT 52和GTGANTG 10。构建了BraA08g014780.3Cp：：GUS表达载体，转化拟南芥Col-0.对转基因拟南芥进行PCR和测序分析，结果表明，由BraA08g014780.3C启动子驱动的GUS基因已成功转化到拟南芥中。GUS染色结果表明，BraA08g014780.3C的启动子为花药特异性启动子。花药特异性启动子的鉴定为揭示BraA08g014780.3C在大白菜雄性不育中的功能奠定了基础。补充信息：在线版本包含补充材料，可在10.1007/s13205-022-03160-z获得。
The promoter of the male sterile gene is important for studying male sterility. In this study,BraA08g014780.3Cwhich differentially expressed between male sterile and fertile plants was identified from a genetic male sterile AB line of Chinese cabbage by RNA-seq. qRT-PCR revealed thatBraA08g014780.3Cwas mainly expressed in the early stage of floral bud development in fertile plants, and preferentially expressed in their anthers. The promoter ofBraA08g014780.3Cwas cloned and analyzed.Cisacting element analysis showed that the promoter ofBraA08g014780.3Ccontains POLLEN1LELAT52 and GTGANTG10, which are both pollen-specific expression elements. TheBraA08g014780.3Cp::GUSvector was constructed, then transformed toArabidopsis thalianaCol-0. PCR analysis and sequencing of the transgenicArabidopsisrevealed that theGUSgene driven by theBraA08g014780.3Cpromoter was successfully transformed to theArabidopsis. GUS staining indicated that the promoter ofBraA08g014780.3Cwas an anther-specific promoter. Identifying the anther-specific promoter laid a foundation for revealingBraA08g014780.3Cfunction in male sterility of Chinese cabbage.Supplementary information:The online version contains supplementary material available at 10.1007/s13205-022-03160-z.
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影响因子：
2.8
作者：
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