Classification of rotavirus VP4 and VP7 serotypes.
Classification of rotavirus VP4 and VP7 serotypes.
复制标题
轮状病毒 VP4 和 VP7 血清型的分类。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
AlbertZ Kapikian
中科院分区：
文献类型：
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作者：
Y. Hoshino;AlbertZ Kapikian
轮状病毒属呼肠孤病毒科，是引起包括人类在内的多种哺乳动物和禽类幼仔急性非细菌性胃肠炎的主要病原体。对轮状病毒胃肠炎的有效免疫预防的需要已经刺激了对这些试剂的生物化学、分子、遗传和临床方面的兴趣，目的是开发安全有效的疫苗。由于中和抗体似乎在针对许多病毒性疾病的保护中发挥重要作用，因此诱导中和抗体的轮状病毒抗原在轮状病毒疫苗的研究和开发中发挥了核心作用。已证明构成完整轮状病毒颗粒的外衣壳的VP7糖蛋白和VP4刺突蛋白是独立的中和抗原。由于轮状病毒的外衣壳蛋白的类型特异性似乎在实验动物模型中对疾病的保护中起重要作用，因此一直在努力对VP7或VP4衣壳蛋白的中和特异性进行分类和分型。基于同源和异源相互中和抗体滴度之间> 20倍差异的标准，已经建立了14种VP7（G）血清型。正在进行研究以表征和分类表现出14种不同G血清型的菌株中的VP4（P）血清型。尝试基于相同的标准（即，> 20倍抗体差异）的方法正在进行中。这种> 20倍抗体差异的标准可以应用于产生具有VP4编码基因（和不相关的VP7编码基因）的重组物的超免疫血清。基因型可以提供分类的线索，但菌株的血清型应基于中和。
Rotaviruses, members of the Reoviridae family, are major etiologic agents of acute nonbacterial gastroenteritis of the young in a wide variety of mammalian and avian species, including humans. The need for effective immunoprophylaxis against rotaviral gastroenteritis has stimulated interest in the biochemical, molecular, genetic, and clinical aspects of these agents with the aim of developing safe and effective vaccines. Because neutralizing antibodies appear to play an important role in protection against many viral diseases, rotavirus antigens that induce neutralizing antibodies have played a central role in research and development of a rotavirus vaccine. The VP7 glycoprotein and VP4 spike protein that constitute the outer capsid of a complete rotavirus particle have been shown to be independent neutralization antigens. Since type specificity of the outer capsid proteins of a rotavirus appears to play an important role in protection against disease in experimental animal models, continued efforts have been made for classification and typing of neutralization specificities on the VP7 or VP4 capsid protein. Based on a criterion of > 20-fold differences between the homologous and heterologous reciprocal neutralizing antibody titers, fourteen VP7 (G) serotypes have been established. Studies are underway to characterize and classify the VP4 (P) serotypes among the strains that exhibit the fourteen different G serotypes. Attempts to classify the VP4 serotypes based on the same criterion (i.e., > 20-fold antibody differences) that is applied to classification of VP7 serotypes are in progress. This standard of > 20-fold antibody differences can be applied with hyperimmune serum raised to a reassortant possessing the VP4 encoding gene (and an unrelated VP7 encoding gene). Genotypes can provide leads towards classification but the serotype of a strain should be based on neutralization.
五种鼠轮状病毒株VP4和VP7的比较。
影响因子：
3.7
作者：
Dunn,SJ;Burns,JW;Cross,TL;Vo,PT;Ward,RL;Bremont,M;Greenberg,HB
通讯作者：
Greenberg,HB