Lack of association between glutathione content and development of thermal tolerance in human fibroblasts.
Lack of association between glutathione content and development of thermal tolerance in human fibroblasts.
复制标题
谷胱甘肽含量与人类成纤维细胞耐热性发展之间缺乏关联。
作者：
M. Lilly;A. Carroll;J. Prchal
热耐受性是细胞和组织在暴露于亚致死热或某些化学物质后发生的一种短暂的耐热状态。虽然这种耐药性的机制尚不清楚，但最近的研究表明，在其形成之前，细胞谷胱甘肽（GSH）水平升高。我们利用谷胱甘肽合成酶缺乏[GSH(-)]的人成纤维细胞系来研究谷胱甘肽含量与耐热性之间的关系。GSH（-）细胞的GSH含量约为正常成纤维细胞的6%。正常成纤维细胞和谷胱甘肽（-）成纤维细胞在暴露于45℃后表现出相似的存活率。将正常成纤维细胞暴露于高温（45℃持续15分钟）会导致细胞谷胱甘肽含量迅速上升，并产生短暂的热耐受性。对GSH（-）成纤维细胞进行类似处理后，极低的GSH水平没有发生变化，但与正常细胞的热耐受程度相似。GSH（-）细胞的热耐受性比正常成纤维细胞衰退得更快。我们得出结论，人成纤维细胞的热耐受性的发展与谷胱甘肽含量无关。
Thermal tolerance is a transient state of heat resistance occurring in cells and tissues after exposure to sublethal heat or certain chemicals. Although the mechanism of such resistance is unknown, it has been recently shown that preceding its development, cellular glutathione (GSH) levels rise. We have used a glutathione synthetase-deficient [GSH(-)] human fibroblast line to study the relationship between glutathione content and thermal tolerance. The GSH(-) cells had approximately 6% as much GSH as normal fibroblasts. Normal and GSH(-) fibroblasts showed similar survival after exposure to 45 degrees C. Exposure of normal fibroblasts to heat (45 degrees C for 15 min) led to a prompt rise in cellular GSH content as well as development of transient thermal tolerance. Similar treatment of GSH(-) fibroblasts produced no change in the very low GSH levels but was associated with a degree of thermal tolerance similar to that of normal cells. Thermal tolerance decayed more rapidly in GSH(-) cells than in normal fibroblasts. We conclude that the development of thermal tolerance in human fibroblasts is independent of GSH content.
多羟基化合物和耐热性：建议的串联。
影响因子：
3.4
作者：
Henle,KJ;Nagle,WA;MossJr,AJ;Herman,TS
通讯作者：
Herman,TS
提出的基于营养物质和初始处理温度的影响的耐热性操作模型。
影响因子：
11.2
作者：
Li,GC;Hahn,GM
通讯作者：
Hahn,GM
DOI：
10.1073/pnas.79.10.3218
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LI, GC;WERB, Z
通讯作者：
WERB, Z