6-gingerdiols as the major metabolites of 6-gingerol in cancer cells and in mice and their cytotoxic effects on human cancer cells.
6-gingerdiols as the major metabolites of 6-gingerol in cancer cells and in mice and their cytotoxic effects on human cancer cells.
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DOI：
10.1021/jf303879b
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发表时间：
2012-11-14
影响因子：
6.1
通讯作者：
Sang S
中科院分区：
文献类型：
--
作者：
Lv L;Chen H;Soroka D;Chen X;Leung T;Sang S
6-姜辣素是姜科植物生姜的主要辛辣成分，具有抗肿瘤活性。然而，6-姜辣素的代谢命运及其代谢产物对所观察到的活性的贡献仍不清楚。在本研究中，我们研究了6-姜辣素在不同的癌细胞和小鼠体内的生物转化，纯化和鉴定了人肺癌细胞的主要代谢产物，并确定了主要代谢产物对人癌细胞增殖的影响。我们的研究结果表明，6-姜酚广泛代谢H-1299人肺癌细胞，CL-13小鼠肺癌细胞，HCT-116和HT-29人结肠癌细胞，并在小鼠。对H-1299细胞中的两个主要代谢产物进行了纯化，并通过1D和2D NMR数据分析，鉴定为（3R，5S）-6-姜二醇（M1）和（3S，5S）-6-姜二醇（M2）。24小时后，两种代谢产物均诱导癌细胞的细胞毒性，其中M1在H-1299细胞中具有与6-姜辣素相当的作用。
6-Gingerol, a major pungent component of ginger (Zingiber officinale Roscoe, Zingiberaceae), has been reported to have anti-tumor activities. However, the metabolic fate of 6-gingerol and the contribution of its metabolites to the observed activities are still unclear. In the present study, we investigated the biotransformation of 6-gingerol in different cancer cells and in mice, purified and identified the major metabolites from human lung cancer cells, and determined the effects of the major metabolites on the proliferation of human cancer cells. Our results show that 6-gingerol is extensively metabolized in H-1299 human lung cancer cells, CL-13 mouse lung cancer cells, HCT-116 and HT-29 human colon cancer cells, and in mice. The two major metabolites in H-1299 cells were purified and identified as (3R,5S)-6-gingerdiol (M1) and (3S,5S)-6-gingerdiol (M2) based on the analysis of their 1D and 2D NMR data. Both metabolites induced cytotoxicity in cancer cells after 24 hours, with M1 having a comparable effect to 6-gingerol in H-1299 cells.
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DOI：
10.1158/1055-9965.epi-07-2934
发表时间：
2008-08
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Zick SM;Djuric Z;Ruffin MT;Litzinger AJ;Normolle DP;Alrawi S;Feng MR;Brenner DE
通讯作者：
Brenner DE
影响因子：
1.8
作者：
Kim, Min Gi;Shin, Beom Soo;Yoo, Sun Dong
通讯作者：
Yoo, Sun Dong
影响因子：
1.7
作者：
NAORA, K;DING, GH;IWAMOTO, K
通讯作者：
IWAMOTO, K
影响因子：
3.8
作者：
KIKUZAKI, H;TSAI, SM;NAKATANI, N
通讯作者：
NAKATANI, N
影响因子：
2
作者：
Shao, Xi;Chen, Xiaoxin;Sang, Shengmin
通讯作者：
Sang, Shengmin