Circulating miR-17, miR-20a, miR-29c, and miR-223 combined as non-invasive biomarkers in nasopharyngeal carcinoma.
Circulating miR-17, miR-20a, miR-29c, and miR-223 combined as non-invasive biomarkers in nasopharyngeal carcinoma.
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循环 miR-17、miR-20a、miR-29c 和 miR-223 组合作为鼻咽癌的非侵入性生物标志物
DOI：
10.1371/journal.pone.0046367
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Peng S
中科院分区：
文献类型：
--
作者：
Zeng X;Xiang J;Wu M;Xiong W;Tang H;Deng M;Li X;Liao Q;Su B;Luo Z;Zhou Y;Zhou M;Zeng Z;Li X;Shen S;Shuai C;Li G;Fang J;Peng S
由于MicroRNAs在血液中的稳定性和在许多疾病中的表达特征，其已被认为是一种潜在的新型癌症检测生物标志物。方法对20例鼻咽癌患者诊断时的血清miRNA进行微阵列分析，并与20例非癌性个体作为对照。随后对30名鼻咽癌患者和30名年龄匹配的非癌志愿者进行实时定量聚合酶链反应（RT-qPCR）。通过对74例鼻咽癌患者和57例非癌志愿者的分析，对数学计算公式的转换建立诊断模型进行了验证。结果39个和17个mirna分别在非癌志愿者和鼻咽癌患者血清中特异表达。发现4种mirna，包括miR-17、miR-20a、miR-29c和miR-223在鼻咽癌患者血清中的表达与非癌对照存在差异。在此基础上，建立了Ct差值法鉴别鼻咽癌病例与非癌对照的诊断方程，并验证了该方程具有较高的敏感性和特异性。结论基于血清mirna的生物标志物模型将成为鼻咽癌检测的新工具。基于循环4- mirna的方法可能为鼻咽癌的诊断提供一种新的策略。
Background MicroRNAs have been considered as a kind of potential novel biomarker for cancer detection due to their remarkable stability in the blood and the characteristics of their expression profile in many diseases. Methods We performed microarray-based serum miRNA profiling on the serum of twenty nasopharyngeal carcinoma patients at diagnosis along with 20 non-cancerous individuals as controls. This was followed by a real-time quantitative Polymerase Chain Reaction (RT-qPCR) in a separate cohort of thirty patients with nasopharyngeal carcinoma and thirty age- matched non-cancerous volunteers. A model for diagnosis was established by a conversion of mathematical calculation formula which has been validated by analyzing 74 cases of patients with nasopharyngeal carcinoma and 57 cases of non-cancerous volunteers. Results The profiles showed that 39 and 17 miRNAs are exclusively expressed in the serum of non-cancerous volunteers and of patients with nasopharyngeal carcinoma respectively. 4 miRNAs including miR-17, miR-20a, miR-29c, and miR-223 were found to be expressed differentially in the serum of NPC compared with that of non-cancerous control. Based on this, a diagnosis equation with Ct difference method has been established to distinguish NPC cases and non-cancerous controls and validated with high sensitivity and specificity. Conclusions We demonstrate that the serum miRNA-based biomarker model become a novel tool for NPC detection. The circulating 4-miRNA-based method may provide a novel strategy for NPC diagnosis.
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