Serum microRNAs are promising novel biomarkers.
Serum microRNAs are promising novel biomarkers.
复制标题
DOI：
10.1371/journal.pone.0003148
复制
发表时间：
2008-09-05
期刊：
影响因子：
3.7
通讯作者：
Chajut A
中科院分区：
文献类型：
--
作者：
Gilad S;Meiri E;Yogev Y;Benjamin S;Lebanony D;Yerushalmi N;Benjamin H;Kushnir M;Cholakh H;Melamed N;Bentwich Z;Hod M;Goren Y;Chajut A
循环核酸(CNA)为临床早期诊断提供了独特的机会。在这里，我们展示了microRNAs，一种参与人类发育和病理的小型非编码调控RNA家族，存在于体液中，代表着新的有效生物标志物。在制定了提取和定量血清和其他体液中microRNA的方案后，我们测定了几个健康人的血清microRNA图谱，发现它们是相似的，验证了我们方法的稳健性。为了解决特定microRNAs丰度在生理或病理条件下可能发生变化的可能性，对怀孕和非怀孕妇女的血清microRNA水平进行了比较。在孕妇血清中，与人胎盘相关的microRNAs显著升高，且其水平与妊娠阶段有关。考虑到microRNAs在发育和疾病中的核心作用，我们的结果强调了测定血清和其他体液中microRNA水平的医学相关潜力。因此，microRNAs是一类新的CNA，有望作为有用的临床生物标记物。
Circulating nucleic acids (CNAs) offer unique opportunities for early diagnosis of clinical conditions. Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarkers. After developing protocols for extracting and quantifying microRNAs in serum and other body fluids, the serum microRNA profiles of several healthy individuals were determined and found to be similar, validating the robustness of our methods. To address the possibility that the abundance of specific microRNAs might change during physiological or pathological conditions, serum microRNA levels in pregnant and non pregnant women were compared. In sera from pregnant women, microRNAs associated with human placenta were significantly elevated and their levels correlated with pregnancy stage. Considering the central role of microRNAs in development and disease, our results highlight the medically relevant potential of determining microRNA levels in serum and other body fluids. Thus, microRNAs are a new class of CNAs that promise to serve as useful clinical biomarkers.
登录
查看更多内容
影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
DOI：
10.1196/annals.1368.001
发表时间：
2006-01-01
期刊：
CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM IV
影响因子：
--
作者：
Swaminathan, R.;Butt, Asif N.
通讯作者：
Butt, Asif N.
影响因子：
2.7
作者：
Shi, R;Chiang, VL
通讯作者：
Chiang, VL
影响因子：
9.3
作者：
Chim, Stephen S. C.;Shing, Tristan K. F.;Lo, Y. M. Dennis
通讯作者：
Lo, Y. M. Dennis