MRI tracking of FePro labeled fresh and cryopreserved long term in vitro expanded human cord blood AC133+ endothelial progenitor cells in rat glioma.
MRI tracking of FePro labeled fresh and cryopreserved long term in vitro expanded human cord blood AC133+ endothelial progenitor cells in rat glioma.
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DOI：
10.1371/journal.pone.0037577
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Arbab AS
中科院分区：
文献类型：
--
作者：
Janic B;Jafari-Khouzani K;Babajani-Feremi A;Iskander AS;Varma NR;Ali MM;Knight RA;Arbab AS
内皮祖细胞（EPC）对于开发各种疾病的细胞疗法非常重要。然而，开发此类疗法的主要障碍是患者体内产生的 EPC 数量较少，并且缺乏足够的非侵入性成像方法来监测移植细胞的体内。该项目的目的是确定脐带血 (CB) AC133+ EPC 在长期体外扩增和冷冻保存后在体外和体内分化为成熟内皮细胞 (EC) 的能力，并使用磁共振成像 (MRI) 评估原位神经胶质瘤大鼠模型中离体扩增和冷冻保存的 CB AC133+ EPC 的体内迁移潜力。原代 CB AC133+ EPC 培养物主要含有 EPC，长期体外条件有利于这些细胞维持内皮谱系的定型状态。在原代培养的第 15-20 天和 25-30 天，细胞用 FePro 标记并冷冻保存几周。将冷冻保存的细胞解冻并进行体外分化或静脉注射给患有神经胶质瘤的大鼠。不同组的大鼠还接受长期培养的、磁性标记的新鲜EPC，并且两组动物在IV施用EPC后7天接受MRI。荧光显微镜显示EPCs的体外分化不受FePro标记和冷冻保存的影响。 MRI 分析表明，与非冷冻保存的细胞相比，先前冷冻保存的移植细胞的体内积累导致 R2 和 R2* 值显着升高，表明先前冷冻保存的 CB AC133+ EPC 向神经胶质瘤部位的迁移和掺入肿瘤新生血管的比率更高。磁性标记的 CB EPC 可以在体外扩增并冷冻保存，以供将来用作 MRI 探针，用于监测新血管形成部位的迁移和掺入。
Endothelial progenitors cells (EPCs) are important for the development of cell therapies for various diseases. However, the major obstacles in developing such therapies are low quantities of EPCs that can be generated from the patient and the lack of adequate non-invasive imaging approach for in vivo monitoring of transplanted cells. The objective of this project was to determine the ability of cord blood (CB) AC133+ EPCs to differentiate, in vitro and in vivo, toward mature endothelial cells (ECs) after long term in vitro expansion and cryopreservation and to use magnetic resonance imaging (MRI) to assess the in vivo migratory potential of ex vivo expanded and cryopreserved CB AC133+ EPCs in an orthotopic glioma rat model. The primary CB AC133+ EPC culture contained mainly EPCs and long term in vitro conditions facilitated the maintenance of these cells in a state of commitment toward endothelial lineage. At days 15–20 and 25–30 of the primary culture, the cells were labeled with FePro and cryopreserved for a few weeks. Cryopreserved cells were thawed and in vitro differentiated or IV administered to glioma bearing rats. Different groups of rats also received long-term cultured, magnetically labeled fresh EPCs and both groups of animals underwent MRI 7 days after IV administration of EPCs. Fluorescent microscopy showed that in vitro differentiation of EPCs was not affected by FePro labeling and cryopreservation. MRI analysis demonstrated that in vivo accumulation of previously cryopreserved transplanted cells resulted in significantly higher R2 and R2* values indicating a higher rate of migration and incorporation into tumor neovascularization of previously cryopreserved CB AC133+ EPCs to glioma sites, compared to non-cryopreserved cells. Magnetically labeled CB EPCs can be in vitro expanded and cryopreserved for future use as MRI probes for monitoring the migration and incorporation to the sites of neovascularization.
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影响因子：
20.3
作者：
Bhattacharya, V;McSweeney, PA;Wu, MHD
通讯作者：
Wu, MHD
影响因子：
2.1
作者：
Dong, Chunming;Goldschmidt-Clermont, Pascal J
通讯作者：
Goldschmidt-Clermont, Pascal J
DOI：
10.1007/bf01476669
发表时间：
1978-01-01
期刊：
KLINISCHE WOCHENSCHRIFT
影响因子：
--
作者：
HLADOVEC, J;PREROVSKY, I;FABIAN, J
通讯作者：
FABIAN, J
影响因子：
20.1
作者：
Fujiyama, S;Amano, K;Matsubara, H
通讯作者：
Matsubara, H
影响因子：
82.9
作者：
Ceradini, DJ;Kulkarni, AR;Gurtner, GC
通讯作者：
Gurtner, GC