Distinct Changes in Calpain and Calpastatin during PNS Myelination and Demyelination in Rodent Models.
Distinct Changes in Calpain and Calpastatin during PNS Myelination and Demyelination in Rodent Models.
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DOI：
10.3390/ijms232315443
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发表时间：
2022-12-06
影响因子：
5.6
通讯作者：
Susuki, Keiichiro
中科院分区：
文献类型：
--
作者：
Miller, John A. A.;Drouet, Domenica E. E.;Yermakov, Leonid M. M.;Elbasiouny, Mahmoud S. S.;Bensabeur, Fatima Z. Z.;Bottomley, Michael;Susuki, Keiichiro
轴突周围形成的髓磷脂提供电绝缘并确保电脉冲的快速有效传输。有髓神经的破坏通常会导致神经传导失败以及神经系统症状和长期残疾。在中枢神经系统中，钙蛋白酶（钙依赖性半胱氨酸蛋白酶家族）已被证明在发育性髓鞘形成和脱髓鞘疾病中发挥作用。钙蛋白酶在周围神经系统髓鞘形成和脱髓鞘中的作用仍不清楚。在这里，我们发现当髓鞘质活跃形成时，出生后大鼠坐骨神经中激活的 CAPN1（一种主要的钙蛋白酶亚型）会短暂增加。内源性钙蛋白酶抑制剂钙蛋白酶抑制素的表达在整个周围神经发育期间呈稳定下降。在以髓鞘形成障碍为特征的 Trembler-J 小鼠的坐骨神经中，CAPN1 和 calpastatin 的表达水平以及 calpain 活性显着增加。在溶血卵磷脂诱导的成年大鼠坐骨神经急性脱髓鞘中，我们发现 CAPN1 表达增加，钙蛋白酶抑制蛋白表达减少。钙蛋白酶-钙蛋白酶抑制素系统中的这些变化与中枢神经系统发育期间或周围神经急性轴突变性期间的变化不同。我们的结果表明钙蛋白酶-钙蛋白酶抑制素系统在周围神经的髓鞘形成和脱髓鞘疾病中具有假定的作用。
Myelin forming around axons provides electrical insulation and ensures rapid and efficient transmission of electrical impulses. Disruptions to myelinated nerves often result in nerve conduction failure along with neurological symptoms and long-term disability. In the central nervous system, calpains, a family of calcium dependent cysteine proteases, have been shown to have a role in developmental myelination and in demyelinating diseases. The roles of calpains in myelination and demyelination in the peripheral nervous system remain unclear. Here, we show a transient increase of activated CAPN1, a major calpain isoform, in postnatal rat sciatic nerves when myelin is actively formed. Expression of the endogenous calpain inhibitor, calpastatin, showed a steady decrease throughout the period of peripheral nerve development. In the sciatic nerves of Trembler-J mice characterized by dysmyelination, expression levels of CAPN1 and calpastatin and calpain activity were significantly increased. In lysolecithin-induced acute demyelination in adult rat sciatic nerves, we show an increase of CAPN1 and decrease of calpastatin expression. These changes in the calpain-calpastatin system are distinct from those during central nervous system development or in acute axonal degeneration in peripheral nerves. Our results suggest that the calpain-calpastatin system has putative roles in myelination and demyelinating diseases of peripheral nerves.
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影响因子：
3.7
作者：
Huff TB;Shi Y;Sun W;Wu W;Shi R;Cheng JX
通讯作者：
Cheng JX
影响因子：
4.7
作者：
Das A;Guyton MK;Smith A;Wallace G 4th;McDowell ML;Matzelle DD;Ray SK;Banik NL
通讯作者：
Banik NL
影响因子：
5.3
作者：
Ewaleifoh, Osefame;Minh Trinh;Griffin, John W.;Thien Nguyen
通讯作者：
Thien Nguyen
影响因子：
25
作者：
Baraban M;Koudelka S;Lyons DA
通讯作者：
Lyons DA
影响因子：
4.4
作者：
BLOMGREN, K;KARLSSON, JO
通讯作者：
KARLSSON, JO