The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction.
The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction.
复制标题
使用半腱肌腱作为自体移植物重建兔半月板的潜力
DOI：
10.1038/s41598-017-07166-z
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发表时间：
2017-08-01
影响因子：
4.6
通讯作者：
Ao Y
中科院分区：
文献类型：
--
作者：
Li C;Hu X;Meng Q;Zhang X;Zhu J;Dai L;Cheng J;Zhong M;Shi W;Ren B;Zhang J;Fu X;Duan X;Ao Y
由于同种异体半月板或人工半月板移植受移植物来源和一系列不良反应的限制，半月板重建的替代方法仍需探索。天然生物材料可以提供独特的三维微环境，是组织工程的一种很有前途的选择。其中，自体移植物因其安全性和良好的生物相容性而成为首选。在本研究中，我们利用自体半腱肌腱移植重建半月板，以探讨其纤维软骨化的潜在性和软骨保护作用。肌腱源性干细胞（Tendon-derived stem cells，TDSCs）和滑膜源性间充质干细胞（synovi-derived mesenchymal stem cells，SMSCs）是本研究中最重要的两种干细胞来源，在脱细胞半腱肌腱（semi-tendinosus tendon，DST）支架上表现出良好的存活、分布、增殖和向纤维软骨细胞分化的能力。肌腱移植物的组织学评价表明，内源性干细胞分化成纤维软骨细胞，合成蛋白多糖，II型胶原和放射状I型胶原在术后12周和24周。移植物的弹性模量和硬度在24周时，自体半月板和再生半月板之间无显著差异。重建组髁突软骨退变的保护作用明显优于对照组。总的来说，自体半腱肌腱移植似乎是一个有前途的半月板重建替代。
Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising alternative for tissue engineering. Among them, autograft is a preferred option for its safety and excellent biocompatibility. In this study, we utilized semitendinosus tendon autograft in meniscus reconstruction to investigate its fibrochondrogenic metaplasticity potential and chondroprotective effect. Tendon-derived stem cells (TDSCs) and synovial-derived mesenchymal stem cells (SMSCs), two most important stem cell sources in our strategy, exhibited excellent viability, distribution, proliferation and fibrochondrogenic differentiation ability in decellularized semitendinosus tendon (DST) scaffoldsin vitro. Histologic evaluation of the tendon graftsin vivosuggested endogenous stem cells differentiated into fibrochondrocytes, synthesized proteoglycan, type II collagen and radial type I collagen at 12 weeks and 24 weeks post-surgery. As for elastic modulus and hardness of the grafts, there were no significant differences between native meniscus and regenerated meniscus at 24 weeks. The protection of condylar cartilage from degeneration was significantly better in the reconstruction group comparing to control group. Overall, semitendinosus tendon autograft seems to be a promising substitution in meniscus reconstruction.
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影响因子：
--
作者：
Ozeki, Nobutake;Muneta, Takeshi;Koga, Hideyuki;Katagiri, Hiroki;Otabe, Koji;Okuno, Makiko;Tsuji, Kunikazu;Kobayashi, Eiji;Matsumoto, Kenji;Saito, Hirohisa;Saito, Tomoyuki;Sekiya, Ichiro
通讯作者：
Sekiya, Ichiro
影响因子：
14
作者：
Huang, Hongjie;Zhang, Xin;Ao, Yingfang
通讯作者：
Ao, Yingfang
影响因子：
4.8
作者：
Patel, Jay M.;Merriam, Aaron R.;Dunn, Michael G.
通讯作者：
Dunn, Michael G.
影响因子：
4.8
作者：
Freedman, KB;D'Amato, MJ;Bach, BR
通讯作者：
Bach, BR
DOI：
10.5604/15093492.1119613
发表时间：
2014-07-01
期刊：
Ortopedia, traumatologia, rehabilitacja
影响因子：
--
作者：
Dziedzic, Dawid;Ciszek, Bogdan
通讯作者：
Ciszek, Bogdan