Optical silencing of C. elegans cells with arch proton pump.
Optical silencing of C. elegans cells with arch proton pump.
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DOI：
10.1371/journal.pone.0035370
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Takagi S
中科院分区：
文献类型：
--
作者：
Okazaki A;Sudo Y;Takagi S
Optogenetic techniques using light-driven ion channels or ion pumps for controlling excitable cells have greatly facilitated the investigation of nervous systems in vivo. A model organism, C. elegans, with its small transparent body and well-characterized neural circuits, is especially suitable for optogenetic analyses. We describe the application of archaerhodopsin-3 (Arch), a recently reported optical neuronal silencer, to C. elegans. Arch::GFP expressed either in all neurons or body wall muscles of the entire body by means of transgenes were localized, at least partially, to the cell membrane without adverse effects, and caused locomotory paralysis of worms when illuminated by green light (550 nm). Pan-neuronal expression of Arch endowed worms with quick and sustained responsiveness to such light. Worms reliably responded to repeated periods of illumination and non-illumination, and remained paralyzed under continuous illumination for 30 seconds. Worms expressing Arch in different subsets of motor neurons exhibited distinct defects in the locomotory behavior under green light: selective silencing of A-type motor neurons affected backward movement while silencing of B-type motor neurons affected forward movement more severely. Our experiments using a heat-shock-mediated induction system also indicate that Arch becomes fully functional only 12 hours after induction and remains functional for more than 24 hour. Arch can be used for silencing neurons and muscles, and may be a useful alternative to currently widely used halorhodopsin (NpHR) in optogenetic studies of C. elegans.
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DOI：
10.1523/jneurosci.4190-10.2010
发表时间：
2010-11-10
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Knöpfel T;Lin MZ;Levskaya A;Tian L;Lin JY;Boyden ES
通讯作者：
Boyden ES
影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
10.5
作者：
Winnier, AR;Meir, JYJ;Miller, DM
通讯作者：
Miller, DM
影响因子：
48
作者：
Guo, Zengcai V.;Hart, Anne C.;Ramanathan, Sharad
通讯作者：
Ramanathan, Sharad
影响因子：
9.2
作者：
Nagel, G;Brauner, M;Gottschalk, A
通讯作者：
Gottschalk, A