Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite.
Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite.
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聚吡咯-透明质酸多壁碳纳米管复合材料的电活性及生物相容性
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
T. Vuorinen
中科院分区：
文献类型：
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作者：
J. Pelto;S. Haimi;Esa Puukilainen;Philip G. Whitten;G. Spinks;M. Bahrami;M. Ritala;T. Vuorinen
用电化学原子力显微镜(EC-AFM)原位研究了氧化碳纳米管电聚合聚吡咯透明质酸(PPyHA-CNT)在生理电解液中的电活性。对生长在AT-Cut 5 MHz石英晶体上的聚合物进行了原位拉曼光谱和石英晶体微天平(QCM)研究。用Live/Dead染色检测人脂肪干细胞(ASC)的贴壁率和存活率，用WST-1细胞增殖实验检测聚吡咯在钛表面的增殖情况。根据循环伏安法，测得该材料在金表面的比电容约为参考聚吡咯十二烷基苯磺酸盐(PPyDBS)的20%。对210 nm厚薄膜的电化学-QCM(EC-QCM)分析表明，该材料非常软，G‘约为100kPa，还原时会膨胀。在微电极上聚合的样品的EC-AFM表明，存在不同的电活性区域，特别是没有疏水背衬PPyDBS层的样品。AFM线扫描显示，在电化学还原过程中，厚度变化通常为20%-25%。拉曼光谱分析表明，该材料支持明显的极化子传导。钛表面的PPyHA-CNT与脂肪干细胞的生物相容性研究表明，与参考的聚乳酸相比，PPyHA-CNT的细胞贴壁率、存活率和增殖率相同或更好。
Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA-CNT) was studied in situ by electrochemical atomic force microscopy (EC-AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT-cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST-1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20% of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical-QCM (EC-QCM) analysis of a 210-nm thick film reveals that the material is very soft G' approximately 100 kPa and swells upon reduction. EC-AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20-25% thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA-CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide.
影响因子：
14
作者：
Peattie, RA;Rieke, ER;Prestwich, GD
通讯作者：
Prestwich, GD