Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions.
Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions.
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小鼠海马体中的 Tomosyn 表达模式表明突触前和突触后功能。
DOI：
10.3389/fnana.2010.00149
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发表时间：
2010
影响因子：
2.9
通讯作者：
Ashery U
中科院分区：
文献类型：
--
作者：
Barak B;Williams A;Bielopolski N;Gottfried I;Okun E;Brown MA;Matti U;Rettig J;Stuenkel EL;Ashery U
Tomosyn蛋白降低突触传递和囊泡释放概率，并且对于调节神经元中的突触传递是必需的。在这项研究中，我们提供了一个详细的描述的表达和定位模式的tomosyn1和tomosyn2在小鼠海马的分区。使用共聚焦和双光子高分辨率显微镜，我们证明，tomosyn共定位与几个前和突触后标记，主要是在mammatergic突触。具体来说，我们发现，tomosyn1是差异分布在小鼠海马，主要集中在门和苔藓纤维。令人惊讶的是，我们发现，tomosyn2表达在下托，CA1和CA2锥体细胞体，树突和棘，并与PSD 95共定位，这表明突触后的作用。这些结果表明，除了tomosyn在神经递质释放的良好特征的突触前功能，tomosyn2可能具有突触后功能，并将tomosyn作为突触传递和可塑性的更一般的调节器。
The protein tomosyn decreases synaptic transmission and release probability of vesicles, and is essential for modulating synaptic transmission in neurons. In this study, we provide a detailed description of the expression and localization patterns of tomosyn1 and tomosyn2 in the subareas of the mouse hippocampus. Using confocal and two-photon high-resolution microscopy we demonstrate that tomosyn colocalizes with several pre- and postsynaptic markers and is found mainly in glutamatergic synapses. Specifically, we show that tomosyn1 is differentially distributed in the mouse hippocampus and concentrated mainly in the hilus and mossy fibers. Surprisingly, we found that tomosyn2 is expressed in the subiculum, CA1 and CA2 pyramidal cell bodies, dendrites and spines, and colocalizes with PSD95, suggesting a postsynaptic role. These results suggest that in addition to the well-characterized presynaptic function of tomosyn in neurotransmitter release, tomosyn2 might have a postsynaptic function, and place tomosyn as a more general regulator of synaptic transmission and plasticity.
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DOI：
10.1083/jcb.200504055
发表时间：
2005-09-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Baba T;Sakisaka T;Mochida S;Takai Y
通讯作者：
Takai Y
影响因子：
2.5
作者：
HAGLUND, L;SWANSON, LW;KOHLER, C
通讯作者：
KOHLER, C
影响因子：
5.3
作者：
Li, Q;Lau, A;Stanley, EF
通讯作者：
Stanley, EF
影响因子：
16.2
作者：
Fujita, Y;Shirataki, H;Takai, Y
通讯作者：
Takai, Y
突触传播中的朋友和敌人：tomosyn在囊泡启动中的作用。
影响因子：
15.9
作者：
Ashery, Uri;Bielopolski, Noa;Barak, Boaz;Yizhar, Ofer
通讯作者：
Yizhar, Ofer