A Highly Selective and Strong Anti-Interference Host-Guest Complex as Fluorescent Probe for Detection of Amantadine by Indicator Displacement Assay.
A Highly Selective and Strong Anti-Interference Host-Guest Complex as Fluorescent Probe for Detection of Amantadine by Indicator Displacement Assay.
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一种高选择性、强抗干扰的主客体复合物作为荧光探针，用于指示剂置换法检测金刚烷胺
DOI：
10.3390/molecules23040947
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发表时间：
2018-04-18
期刊：
影响因子：
--
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Zhu L;Zhao Z;Zhang X;Zhang H;Liang F;Liu S
金刚烷胺 (AMA) 及其衍生物是非法兽药，在极低浓度下很难检测到。迫切需要开发一种快速、简单且高灵敏度的 AMA 检测方法。在这里，我们设计了一种蒽环化合物（ABAM），它与葫芦[7]脲（CB[7]）主体结合，缔合常数高达8.7 × 108 M−1。然后将主客体复合物用作荧光探针来检测 AMA。 AMA 竞争占据 CB[7] 的空腔，使得 ABAM 从 CB[7]-ABAM 复合物中释放，导致 ABAM 显着的荧光猝灭（指示剂置换测定，IDA）。该方法的线性范围为0.000188至0.375 μg/mL，检测限可低至6.5 × 10−5 μg/mL (0.35 nM)。最重要的是，由于CB[7]和ABAM之间的高结合亲和力，该荧光主客体系统表现出很强的抗干扰能力。因此，我们能够准确测定各种样品（包括药物制剂）中 AMA 的浓度。
Amantadine (AMA) and its derivatives are illicit veterinary drugs that are hard to detect at very low concentrations. Developing a fast, simple and highly sensitive method for the detection of AMA is highly in demand. Here, we designed an anthracyclic compound (ABAM) that binds to a cucurbit[7]uril (CB[7]) host with a high association constant of up to 8.7 × 108 M−1. The host-guest complex was then used as a fluorescent probe for the detection of AMA. Competition by AMA for occupying the cavity of CB[7] allows ABAM to release from the CB[7]-ABAM complex, causing significant fluorescence quenching of ABAM (indicator displacement assay, IDA). The linear range of the method is from 0.000188 to 0.375 μg/mL, and the detection limit can be as low as 6.5 × 10−5 μg/mL (0.35 nM). Most importantly, due to the high binding affinity between CB[7] and ABAM, this fluorescence host-guest system shows great anti-interference capacity. Thus, we are able to accurately determine the concentration of AMA in various samples, including pharmaceutical formulations.
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DOI：
10.1016/j.jchromb.2013.09.035
发表时间：
2013-12-01
影响因子：
3
作者：
Farajzadeh, Mir Ali;Nouri, Nina;Nabil, Ali Akbar Alizadeh
通讯作者：
Nabil, Ali Akbar Alizadeh
影响因子：
2
作者：
Dobrovolny, Hana M.;Gieschke, Ronald;Beauchemin, Catherine A. A.
通讯作者：
Beauchemin, Catherine A. A.
扭曲的葫芦[n]urils
影响因子：
5.2
作者：
Li, Qing;Qiu, Sheng-Chao;Wei, Gang
通讯作者：
Wei, Gang
影响因子：
15
作者：
Moghaddam S;Yang C;Rekharsky M;Ko YH;Kim K;Inoue Y;Gilson MK
通讯作者：
Gilson MK
影响因子：
1.6
作者：
Jalali, F.;Maghooli, R.
通讯作者：
Maghooli, R.