Time resolved studies of interfacial reactions of ozone with pulmonary phospholipid surfactants using field induced droplet ionization mass spectrometry.
Time resolved studies of interfacial reactions of ozone with pulmonary phospholipid surfactants using field induced droplet ionization mass spectrometry.
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DOI：
10.1021/jp102332g
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发表时间：
2010-07-29
影响因子：
3.3
通讯作者：
Beauchamp, J. L.
中科院分区：
文献类型：
--
作者：
Kim, Hugh I.;Kim, Hyungjun;Shin, Young Shik;Beegle, Luther W.;Goddard, William A.;Heath, James R.;Kanik, Isik;Beauchamp, J. L.
场致液滴电离质谱（FIDI-MS）包括一种软电离方法，用于从微升液滴表面采样离子。脉冲电场拉伸中性液滴，直到它们形成双泰勒锥，以相反的方向发射带正电荷和负电荷的亚微米液滴流，所需的极性被引导到质谱仪中进行分析。采用负离子模式，研究了1-棕榈酰-2-油酰-sn-磷脂酰甘油（POPG）在气液界面上的非均相臭氧分解反应。我们的研究结果表明，在空气-液体界面的非均相反应的独特特征。我们观察到羟基过氧化氢和二级臭氧化物作为POPG臭氧分解的主要产物在FIDI-MS光谱。这些产物是亚稳态的，难以在本体相中观察到，使用标准电喷雾电离（ESI）进行质谱分析。我们还提出了在空气-液体界面的饱和和不饱和磷脂的混合物的非均相臭氧分解的研究。用负离子模式研究了饱和磷脂1，2-二棕榈酰-sn-磷脂酰甘油（DPPG）和不饱和POPG的混合物，用正离子模式研究了1，2-二棕榈酰-sn-磷脂酰胆碱（DPPC）和1-硬脂酰-2-油酰-sn-磷脂酰胆碱（SOPC）的混合物。在这两种情况下，FIDI-MS显示饱和和不饱和的肺表面活性剂形成混合界面层。只有不饱和磷脂与臭氧反应，形成比饱和磷脂更亲水的产物。随着广泛的臭氧分解，只有饱和磷脂保留在液滴表面。结合这些实验观察与计算分析的结果提供了一个更好的理解的界面结构和化学的表面活性剂层系统时，受到氧化应激。
Field induced droplet ionization mass spectrometry (FIDI-MS) comprises a soft ionization method to sample ions from the surface of microliter droplets. A pulsed electric field stretches neutral droplets until they develop dual Taylor cones, emitting streams of positively and negatively charged submicrometer droplets in opposite directions, with the desired polarity being directed into a mass spectrometer for analysis. This methodology is employed to study the heterogeneous ozonolysis of 1-palmitoyl-2-oleoyl-sn-phosphatidylglycerol (POPG) at the air–liquid interface in negative ion mode using FIDI mass spectrometry. Our results demonstrate unique characteristics of the heterogeneous reactions at the air–liquid interface. We observe the hydroxyhydroperoxide and the secondary ozonide as major products of POPG ozonolysis in the FIDI-MS spectra. These products are metastable and difficult to observe in the bulk phase, using standard electrospray ionization (ESI) for mass spectrometric analysis. We also present studies of the heterogeneous ozonolysis of a mixture of saturated and unsaturated phospholipids at the air–liquid interface. A mixture of the saturated phospholipid 1,2-dipalmitoyl-sn-phosphatidylglycerol (DPPG) and unsaturated POPG is investigated in negative ion mode using FIDI-MS while a mixture of 1,2-dipalmitoyl-sn-phosphatidylcholine (DPPC) and 1-stearoyl-2-oleoyl-sn-phosphatidylcholine (SOPC) surfactant is studied in positive ion mode. In both cases FIDI-MS shows the saturated and unsaturated pulmonary surfactants form a mixed interfacial layer. Only the unsaturated phospholipid reacts with ozone, forming products that are more hydrophilic than the saturated phospholipid. With extensive ozonolysis only the saturated phospholipid remains at the droplet surface. Combining these experimental observations with the results of computational analysis provides an improved understanding of the interfacial structure and chemistry of a surfactant layer system when subject to oxidative stress.
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影响因子：
--
作者：
Marten, B;Kim, K;Honig, B
通讯作者：
Honig, B
影响因子：
4.4
作者：
BECKE, AD
通讯作者：
BECKE, AD
影响因子：
4.1
作者：
PLIMPTON, S
通讯作者：
PLIMPTON, S
DOI：
10.1159/000056765
发表时间：
2002-01-01
期刊：
BIOLOGY OF THE NEONATE
影响因子：
--
作者：
Pérez-Gil, J
通讯作者：
Pérez-Gil, J
DOI：
10.1073/pnas.0710791105
发表时间：
2008-05-27
影响因子：
11.1
作者：
Enami, Shinichi;Hoffmann, M. R.;Colussi, A. J.
通讯作者：
Colussi, A. J.