Identification of Urine Metabolites as Biomarkers of Early Lyme Disease.
Identification of Urine Metabolites as Biomarkers of Early Lyme Disease.
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DOI：
10.1038/s41598-018-29713-y
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发表时间：
2018-08-15
影响因子：
4.6
通讯作者：
Molins CR
中科院分区：
文献类型：
--
作者：
Pegalajar-Jurado A;Fitzgerald BL;Islam MN;Belisle JT;Wormser GP;Waller KS;Ashton LV;Webb KJ;Delorey MJ;Clark RJ;Molins CR
在人类生物体液中可检测到的代谢物是用于诊断早期莱姆病（ELD）（一种媒介传播的传染病）的有吸引力的生物标志物。尿液是一种容易获得的临床样本，可用于诊断目的。然而，很少有研究探索尿液中的ELD生物标志物。在这项研究中，代谢组学方法被应用于评价ELD患者（n = 14）、传染性单核细胞增多症患者（n = 14）和健康对照组（n = 14）尿液中的小分子代谢物。使用非靶向代谢组学生成ELD与健康对照和ELD与传染性单核细胞增多症的代谢生物特征。途径分析和代谢物鉴定显示，与健康对照或单核细胞增多症相比，ELD中的几种代谢过程失调，包括色氨酸代谢。线性判别分析表明，个人的代谢生物特征可以正确区分ELD从其他患者群体的准确性为71至100%。这些数据为使用尿液代谢物作为ELD诊断分类的生物标志物提供了概念验证。
Metabolites detectible in human biofluids are attractive biomarkers for the diagnosis of early Lyme disease (ELD), a vector-borne infectious disease. Urine represents an easily obtained clinical sample that can be applied for diagnostic purposes. However, few studies have explored urine for biomarkers of ELD. In this study, metabolomics approaches were applied to evaluate small molecule metabolites in urine from patients with ELD (n = 14), infectious mononucleosis (n = 14) and healthy controls (n = 14). Metabolic biosignatures for ELD versus healthy controls and ELD versus infectious mononucleosis were generated using untargeted metabolomics. Pathway analyses and metabolite identification revealed the dysregulation of several metabolic processes in ELD as compared to healthy controls or mononucleosis, including metabolism of tryptophan. Linear discriminant analyses demonstrated that individual metabolic biosignatures can correctly discriminate ELD from the other patient groups with accuracies of 71 to 100%. These data provide proof-of-concept for use of urine metabolites as biomarkers for diagnostic classification of ELD.
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影响因子：
4.6
作者：
Fernández-Peralbo MA;Gómez-Gómez E;Calderón-Santiago M;Carrasco-Valiente J;Ruiz-García J;Requena-Tapia MJ;Luque de Castro MD;Priego-Capote F
通讯作者：
Priego-Capote F
影响因子：
--
作者：
Gaines, Linda G. T.;Fent, Kenneth W.;Nylander-French, Leena A.
通讯作者：
Nylander-French, Leena A.
影响因子：
10.6
作者：
Capuron, Lucile;Schroecksnadel, Sebastian;Fuchs, Dietmar
通讯作者：
Fuchs, Dietmar
影响因子：
--
作者：
Saez-Lara MJ;Gomez-Llorente C;Plaza-Diaz J;Gil A
通讯作者：
Gil A
影响因子：
15.1
作者：
Felger, Jennifer C.;Li, Li;Marvar, Paul J.;Woolwine, Bobbi J.;Harrison, David G.;Raison, Charles L.;Miller, Andrew H.
通讯作者：
Miller, Andrew H.