Sonic hedgehog in the notochord is sufficient for patterning of the intervertebral discs.
Sonic hedgehog in the notochord is sufficient for patterning of the intervertebral discs.
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DOI：
10.1016/j.mod.2012.07.003
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发表时间：
2012-09
影响因子：
2.6
通讯作者：
Harfe, Brian D.
中科院分区：
文献类型：
--
作者：
Choi, Kyung-Suk;Lee, Chanmi;Harfe, Brian D.
位于相邻椎骨之间的椎间盘对于脊柱的稳定性和在整个脊柱中分布机械负荷是必需的。位于椎间盘中间区域的所有细胞类型，称为髓核，都来源于胚胎脊索。最近的研究表明，hedgehog信号通路在髓核的形成过程中起着至关重要的作用。然而，在髓核形成期间，Shh在脊索和附近的底板中表达。为了确定足够形成髓核的SHH蛋白的来源，我们使用他莫昔芬诱导的Cre等位基因从底板或脊索中去除Shh。从底板上去除Shh导致表型正常的椎间盘，表明该组织中的Shh表达不是椎间盘图案所需的。此外，在底板中缺乏Shh的胚胎具有正常的脊柱，表明脊索中的Shh表达足以使整个脊柱图案化。从脊索中去除Shh导致底板中Shh的缺失、椎间盘和椎骨结构的丢失。这些数据表明，Shh在脊索中的表达足以形成椎间盘和脊柱的图案。
The intervertebral discs, located between adjacent vertebrae, are required for stability of the spine and distributing mechanical load throughout the vertebral column. All cell types located in thes middle regions of the discs, called nuclei pulposi, are derived from the embryonic notochord. Recently, it was shown that the hedgehog signaling pathway plays an essential role during formation of nuclei pulposi. However, during the time that nuclei pulposi are forming, Shh is expressed in both the notochord and the nearby floor plate. To determine the source of SHH protein sufficient for formation of nuclei pulposi we removed Shh from either the floor plate or the notochord using tamoxifen-inducible Cre alleles. Removal of Shh from the floor plate resulted in phenotypically normal intervertebral discs, indicating that Shh expression in this tissue is not required for disc patterning. In addition, embryos that lacked Shh in the floor plate had normal vertebral columns, demonstrating that Shh expression in the notochord is sufficient for pattering the entire vertebral column. Removal of Shh from the notochord resulted in the absence of Shh in the floor plate, loss of intervertebral discs and vertebral structures. These data indicate that Shh expression in the notochord is sufficient for patterning of the intervertebral discs and the vertebral column.
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影响因子：
2.5
作者：
Choi, Kyung-Suk;Cohn, Martin J.;Harfe, Brian D.
通讯作者：
Harfe, Brian D.
使用 Noto-cre 小鼠追踪脊索衍生细胞：对椎间盘发育的影响。
影响因子：
4.3
作者：
McCann MR;Tamplin OJ;Rossant J;Séguin CA
通讯作者：
Séguin CA
影响因子：
2.5
作者：
Park, Eon Joo;Sun, Xiaoxia;Moon, Anne M.
通讯作者：
Moon, Anne M.
影响因子：
4.3
作者：
Smith LJ;Nerurkar NL;Choi KS;Harfe BD;Elliott DM
通讯作者：
Elliott DM
影响因子：
64.5
作者：
ROELINK, H;PORTER, JA;JESSELL, TM
通讯作者：
JESSELL, TM