Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice.
Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice.
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DOI：
10.1186/ar1865
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发表时间：
2006
影响因子：
4.9
通讯作者：
Conde C
中科院分区：
文献类型：
--
作者：
García S;Bodaño A;González A;Forteza J;Gómez-Reino JJ;Conde C
多聚腺苷二磷酸核糖聚合酶-1（PARP-1）是一种核DNA结合蛋白，参与调节DNA修复和维持基因组完整性。此外，PARP-1在几种炎症疾病模型中发挥作用，其缺失或失活可提供保护。本研究的目的是分析选择性PARP-1抑制对胶原抗体诱导的关节炎的影响。我们发现，PARP-1缺乏部分降低关节炎的严重程度，虽然疾病的发病率是类似的控制和缺陷小鼠。临床评分降低伴随组织病理学结果部分减少。有趣的是，定量实时PCR和ELISA分析显示，PARP-1的缺失下调了关节炎关节中IL-1β和单核细胞趋化蛋白1的表达，而肿瘤坏死因子-α的转录没有受损。我们的研究结果提供了PARP-1对关节炎进展的贡献的证据，并将该蛋白鉴定为治疗类风湿性关节炎的潜在治疗靶点。
Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear DNA-binding protein that participates in the regulation of DNA repair and maintenance of genomic integrity. In addition, PARP-1 has a role in several models of inflammation disease, where its absence or inactivation confers protection. The aim of this study was to analyze the impact of selective PARP-1 suppression in collagen antibody-induced arthritis. We show that PARP-1 deficiency partially reduces the severity of arthritis, although the incidence of disease was similar in control and deficient mice. Decreased clinical scores were accompanied by partial reduction of histopathological findings. Interestingly, quantitative real-time PCR and ELISA analysis revealed that the absence of PARP-1 down-regulated IL-1β and monocyte chemotactic protein 1 expression in arthritic joints whereas tumor necrosis factor-α transcription was not impaired. Our results provide evidence of the contribution of PARP-1 to the progression of arthritis and identify this protein as a potential therapeutic target for the treatment of rheumatoid arthritis.
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DOI：
10.1084/jem.191.2.313
发表时间：
2000-01-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Horai R;Saijo S;Tanioka H;Nakae S;Sudo K;Okahara A;Ikuse T;Asano M;Iwakura Y
通讯作者：
Iwakura Y
影响因子：
--
作者：
García-Vicuña, R;Gómez-Gaviro, MV;Díaz-González, F
通讯作者：
Díaz-González, F
影响因子：
4.8
作者：
Chang, WJ;Alvarez-Gonzalez, R
通讯作者：
Alvarez-Gonzalez, R
影响因子：
158.5
作者：
Moreland, LW;Baumgartner, SW;Blosch, CM
通讯作者：
Blosch, CM
影响因子：
3.7
作者：
Hassa, PO;Hottiger, MO
通讯作者：
Hottiger, MO