The mitochondrial genome of the glomeromycete Rhizophagus sp. DAOM 213198 reveals an unusual organization consisting of two circular chromosomes.
The mitochondrial genome of the glomeromycete Rhizophagus sp. DAOM 213198 reveals an unusual organization consisting of two circular chromosomes.
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DOI：
10.1093/gbe/evu268
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发表时间：
2014-12-19
影响因子：
3.3
通讯作者：
Hijri M
中科院分区：
文献类型：
--
作者：
Nadimi M;Stefani FO;Hijri M
线粒体（mt）基因组在子囊菌门和担子菌门中得到了广泛的研究，但在基础真菌谱系中的记录却很少。在这项研究中，我们测定了Rhizophagus sp. DAOM 213198的mtDNA全序列，它是Rhizophagus irregularis的近亲，Rhizophagus irregularis是一种广泛分布的生态和经济相关物种，属于球囊菌门。与所有其他已知的具有全长环状染色体的分类学上的近亲不同，Rhizophagus sp.的mtDNA显示了一种不寻常的组织，具有两条61，964和29，078 bp的环状染色体。这条大染色体包含9个蛋白质编码基因（atp 9，nad 5，cob，nad 4，nad 1，nad 4L，cox 1，cox 2，atp 8），小亚基rRNA基因（rns），含有20个tRNA编码基因和10个ORF，而小染色体含有5个蛋白质编码基因（atp 6、nad 2、nad 3、nad 6和cox 3）、大亚基rRNA基因（rnl）以及5种tRNA编码基因和8种质粒相关DNA聚合酶（dpo）。虽然植物mt基因组的结构变异已经有了很好的记录，但这项研究是首次报道丛枝菌根真菌中存在两个环状mt基因组。有趣的是，dpo的存在下，在断裂点的intergene cox 1-cox 2和rnl-atp 6的大和小的mtDNA，分别，可能是负责转化为两条染色体的Rhizophagus sp. mtDNA。使用定量实时聚合酶链反应，我们发现，这两个mtDNA具有相同的丰度。这项研究报告了一种新的线粒体DNA组织在球囊菌门，并强调了研究早期不同的真菌谱系，以描述新的进化途径在真菌王国的重要性。
Mitochondrial (mt) genomes are intensively studied in Ascomycota and Basidiomycota, but they are poorly documented in basal fungal lineages. In this study, we sequenced the complete mtDNA of Rhizophagus sp. DAOM 213198, a close relative to Rhizophagus irregularis, a widespread, ecologically and economical relevant species belonging to Glomeromycota. Unlike all other known taxonomically close relatives harboring a full-length circular chromosome, mtDNA of Rhizophagus sp. reveals an unusual organization with two circular chromosomes of 61,964 and 29,078 bp. The large chromosome contained nine protein-coding genes (atp9, nad5, cob, nad4, nad1, nad4L, cox1, cox2, and atp8), small subunit rRNA gene (rns), and harbored 20 tRNA-coding genes and 10 orfs, while the small chromosome contained five protein-coding genes (atp6, nad2, nad3, nad6, and cox3), large subunit rRNA gene (rnl) in addition to 5 tRNA-coding genes, and 8 plasmid-related DNA polymerases (dpo). Although structural variation of plant mt genomes is well documented, this study is the first report of the presence of two circular mt genomes in arbuscular mycorrhizal fungi. Interestingly, the presence of dpo at the breakage point in intergenes cox1-cox2 and rnl-atp6 for large and small mtDNAs, respectively, could be responsible for the conversion of Rhizophagus sp. mtDNA into two chromosomes. Using quantitative real-time polymerase chain reaction, we found that both mtDNAs have an equal abundance. This study reports a novel mtDNA organization in Glomeromycota and highlights the importance of studying early divergent fungal lineages to describe novel evolutionary pathways in the fungal kingdom.
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影响因子：
3.5
作者：
Chen H;Chan DC
通讯作者：
Chan DC
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作者：
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通讯作者：
Lang BF
影响因子：
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作者：
Beaudet D;Terrat Y;Halary S;de la Providencia IE;Hijri M
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Hijri M
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作者：
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作者：
Krueger, Manuela;Krueger, Claudia;Schuessler, Arthur
通讯作者：
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