Mitochondrial genome rearrangements in glomus species triggered by homologous recombination between distinct mtDNA haplotypes.
Mitochondrial genome rearrangements in glomus species triggered by homologous recombination between distinct mtDNA haplotypes.
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DOI：
10.1093/gbe/evt120
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发表时间：
2013
影响因子：
3.3
通讯作者：
Hijri M
中科院分区：
文献类型：
--
作者：
Beaudet D;Terrat Y;Halary S;de la Providencia IE;Hijri M
丛枝菌根真菌(AMF)的比较线粒体基因组学为克服长期障碍提供了新的途径，这些障碍阻碍了旨在了解这些多核和遗传多态生物的群落结构、多样性和进化的研究。AMF线粒体(Mt)基因组在分离株中是同源的，其基因间隔区含有大量迅速分化的可移动元件，包括归巢内切酶基因、小反向重复序列和与质粒相关的DNA聚合酶基因(DPO)，使它们适合于开发可靠的菌株特异性标记。然而，这些元素也可能通过同源重组导致基因组重排，尽管这在这组专性共生真菌中以前从未报道过。为了研究AMF中是否存在这种重排以及这种重排是否是由可移动元件引起的，本文对两个Glomus cerebriforme和Glomus sp.并与现有的肾菌线粒体基因组进行了比较。我们使用一种广泛的基于核苷酸/蛋白质相似性网络的方法来研究AMF以及其他公开可获得序列的生物中DPO的多样性。我们提供了DPO诱导的单倍型间重组的有力证据，导致了Glomus sp.线粒体基因组的重组。这些发现提出了这样一个问题：AMF单孢子培养是否人为地低估了线粒体DNA的遗传多样性。我们评估了潜在的DPO在AMF中的扩散机制，并推断了与植物线粒体质粒之间的系统发育关系。与其他间接证据一起，我们的分析表明，球菌门的成员是植物线粒体质粒的潜在供体。
Comparative mitochondrial genomics of arbuscular mycorrhizal fungi (AMF) provide new avenues to overcome long-lasting obstacles that have hampered studies aimed at understanding the community structure, diversity, and evolution of these multinucleated and genetically polymorphic organisms. AMF mitochondrial (mt) genomes are homogeneous within isolates, and their intergenic regions harbor numerous mobile elements that have rapidly diverged, including homing endonuclease genes, small inverted repeats, and plasmid-related DNA polymerase genes (dpo), making them suitable targets for the development of reliable strain-specific markers. However, these elements may also lead to genome rearrangements through homologous recombination, although this has never previously been reported in this group of obligate symbiotic fungi. To investigate whether such rearrangements are present and caused by mobile elements in AMF, the mitochondrial genomes from two Glomeraceae members (i.e., Glomus cerebriforme and Glomus sp.) with substantial mtDNA synteny divergence, were sequenced and compared with available glomeromycotan mitochondrial genomes. We used an extensive nucleotide/protein similarity network-based approach to investigate dpo diversity in AMF as well as in other organisms for which sequences are publicly available. We provide strong evidence of dpo-induced inter-haplotype recombination, leading to a reshuffled mitochondrial genome in Glomus sp. These findings raise questions as to whether AMF single spore cultivations artificially underestimate mtDNA genetic diversity. We assessed potential dpo dispersal mechanisms in AMF and inferred a robust phylogenetic relationship with plant mitochondrial plasmids. Along with other indirect evidence, our analyses indicate that members of the Glomeromycota phylum are potential donors of mitochondrial plasmids to plants.
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影响因子：
3.3
作者：
Burger G;Gray MW;Forget L;Lang BF
通讯作者：
Lang BF
影响因子：
5.6
作者：
AKINS, RA;GRANT, DM;LAMBOWITZ, AM
通讯作者：
LAMBOWITZ, AM
影响因子：
64.8
作者：
COLLINS, RA;SAVILLE, BJ
通讯作者：
SAVILLE, BJ
影响因子：
9.4
作者：
Croll, Daniel;Giovannetti, Manuela;Sanders, Ian R.
通讯作者：
Sanders, Ian R.
影响因子：
3
作者：
Bader, GD;Hogue, CW
通讯作者：
Hogue, CW