Chitin-based scaffolds are an integral part of the skeleton of the marine demosponge Ianthella basta.
Chitin-based scaffolds are an integral part of the skeleton of the marine demosponge Ianthella basta.
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DOI：
10.1016/j.jsb.2009.06.018
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发表时间：
2009-12
影响因子：
3
通讯作者：
Born, R.
中科院分区：
文献类型：
--
作者：
Brunner, E.;Ehrlich, H.;Schupp, P.;Hedrich, R.;Hunoldt, S.;Kammer, M.;Machill, S.;Paasch, S.;Bazhenov, V. V.;Kurek, D. V.;Arnold, T.;Brockmann, S.;Ruhnow, M.;Born, R.
蠕形虫的骨骼，如巴斯塔棘藻，已知是一种含有有机成分的复合材料。在这里，我们表明，丝状几丁质基支架是I.basa骨架的组成部分。使用基于碱性溶液处理的分离和纯化技术，可以从海绵骨架中分离出这些甲壳素基支架。固体~(13)C核磁共振、拉曼和FT-IR光谱以及几丁质酶消化表明分离的物质确实是由几丁质组成的。用光学显微镜和电子显微镜对支架的形貌进行了测定。它由直径约40-100 nm的交联甲壳素纤维组成，形成一个微结构的网络结构。该网络的整体形状非常类似于整体海绵骨架的形状。固体~(13)C核磁共振波谱在分子水平上对海绵骨架进行了表征。甲壳素支架的~(13)C核磁共振信号相对较宽，表明甲壳素中含有大量无序的甲壳素，可能以表面暴露的分子形式存在。X-射线衍射仪证实，从巴斯塔中分离得到的支架由部分无序、松散堆积的甲壳素组成，表面较大。这些甲壳质支架的光谱特征更接近于α-甲壳素，而不是β-甲壳素。
The skeletons of demosponges, such as Ianthella basta, are known to be a composite material containing organic constituents. Here, we show that a filigree chitin-based scaffold is an integral component of the I. basta skeleton. These chitin-based scaffolds can be isolated from the sponge skeletons using an isolation and purification technique based on treatment with alkaline solutions. Solid-state 13C NMR, Raman, and FT-IR spectroscopies, as well as chitinase digestion, reveal that the isolated material indeed consists of chitin. The morphology of the scaffolds has been determined by light and electron microscopy. It consists of cross-linked chitin fibers approximately 40–100 nm in diameter forming a micro-structured network. The overall shape of this network closely resembles the shape of the integer sponge skeleton. Solid-state 13C NMR spectroscopy was used to characterize the sponge skeleton on a molecular level. The 13C NMR signals of the chitin-based scaffolds are relatively broad, indicating a high amount of disordered chitin, possibly in the form of surface-exposed molecules. X-ray diffraction confirms that the scaffolds isolated from I. basta consist of partially disordered and loosely packed chitin with large surfaces. The spectroscopic signature of these chitin-based scaffolds is closer to that of α-chitin than β-chitin.
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影响因子：
7.4
作者：
FERGUSON, DB;HAW, JF
通讯作者：
HAW, JF
DOI：
10.3891/acta.chem.scand.37b-0267
发表时间：
1983-01-01
期刊：
ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY
影响因子：
--
作者：
HERTZBERG, S;RAMDAHL, T;LIAAENJENSEN, S
通讯作者：
LIAAENJENSEN, S
影响因子：
1.6
作者：
HERTZBERG, S;BERGQUIST, P;LIAAENJENSEN, S
通讯作者：
LIAAENJENSEN, S
影响因子：
2.7
作者：
HUMPHREYS, T
通讯作者：
HUMPHREYS, T
影响因子：
6.2
作者：
Fan, Yimin;Saito, Tsuguyuki;Isogai, Akira
通讯作者：
Isogai, Akira