Cellular origins and lineage relationships of the intestinal epithelium.
Cellular origins and lineage relationships of the intestinal epithelium.
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DOI：
10.1152/ajpgi.00188.2021
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发表时间：
2021-10-01
期刊：
影响因子：
--
通讯作者：
Yan KS
中科院分区：
文献类型：
--
作者：
Capdevila C;Trifas M;Miller J;Anderson T;Sims PA;Yan KS
了解组织的发育和分层组织是理解它们在损伤和疾病中是如何受到干扰的关键，也是理解如何通过治疗操纵它们来恢复体内平衡的关键。快速再生的肠上皮含有多种细胞类型，它们的谱系关系已经用许多方法进行了研究，从经典的标签保留和遗传谱系追踪方法到新的基于转录组的注释。在这里，我们描述发育轨迹，指示分化和谱系规范肠上皮。我们关注最新的基于单细胞rna测序（scRNA-seq）的策略来理解肠上皮细胞谱系关系，强调它们如何改进了我们对该组织发展的看法，并强调了它们的优势和局限性。我们强调如何应用这些技术来理解肠上皮细胞在稳态和损伤诱导再生模型中的动力学。
Knowledge of the development and hierarchical organization of tissues is key to understanding how they are perturbed in injury and disease, as well as how they may be therapeutically manipulated to restore homeostasis. The rapidly regenerating intestinal epithelium harbors diverse cell types and their lineage relationships have been studied using numerous approaches, from classical label-retaining and genetic lineage tracing methods to novel transcriptome-based annotations. Here, we describe the developmental trajectories that dictate differentiation and lineage specification in the intestinal epithelium. We focus on the most recent single-cell RNA-sequencing (scRNA-seq)-based strategies for understanding intestinal epithelial cell lineage relationships, underscoring how they have refined our view of the development of this tissue and highlighting their advantages and limitations. We emphasize how these technologies have been applied to understand the dynamics of intestinal epithelial cells in homeostatic and injury-induced regeneration models.
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