Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients
Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients
复制标题
艾滋病和艾滋病风险患者血清中和人类 T 淋巴细胞病毒 III 型
作者：
R. Weiss;P. Clapham;R. Cheingsong;A. Dalgleish;C. Carne;I. Weller;R. Tedder
人类嗜T淋巴细胞病毒III型（LAV，HTLV-III）1，2在病因学上与获得性免疫缺陷综合征（AIDS）和持续性全身淋巴结病（PGL）相关2-7。特异性放射免疫测定法（RIA）、酶联测定法、免疫荧光测定法（IFA）和免疫印迹技术正被广泛用于检测感染患者和有感染风险的患者中HTLV-III的血清抗体4 -7。然而，这些测定不能在功能上鉴定那些中和病毒感染性的抗体。我们已经使用了三种滴定血清中和因子的方法：抑制合胞体诱导，中和水泡性口炎病毒（VSV）的包膜假型和减少HTLV-III的感染性的细胞系允许病毒复制。我们在这里报告，血清从受试者在不同的疾病类别只有低水平的中和活性，即使抗体的病毒膜抗原是在高滴度。从三个不同国家生产的四种HTLV-III分离株制备的包膜假型对阳性血清的中和同样敏感，包括来自产生两种病毒分离株的患者的血清。
Human T-lymphotropic virus type III (LAV, HTLV-III)1,2 is aetiologically linked to acquired immune deficiency syndrome (AIDS) and persistent general lymphadenopathy (PGL)2–7. Specific radioimmunoassays (RIA), enzyme-linked assays, immunofluorescence assays (IFA) and immunoblotting techniques are being used widely to detect serum antibodies to HTLV-III in infected patients and in those at risk of infection4–7. However, these assays do not functionally identify those antibodies that neutralize the infectivity of the virus. We have used three methods of titrating serum neutralizing factors: inhibition of syncytium induction, neutralization of envelope pseudotypes of vesicular stomatitis virus (VSV) and reduction of infectivity of HTLV-III for a cell line permissive to virus replication. We report here that sera from subjects in various disease categories possess only low-level neutralizing activity, even when antibodies to viral membrane antigens are present in high titre. Envelope pseudotypes prepared from four HTLV-III isolates made in three different countries are equally sensitive to neutralization by positive sera, including sera from patients yielding two of the virus isolates.
影响因子：
56.9
作者：
GONDA, MA;WONGSTAAL, F;GILDEN, RV
通讯作者：
GILDEN, RV
DOI：
10.1073/pnas.79.14.4395
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MEUER, SC;SCHLOSSMAN, SF;REINHERZ, EL
通讯作者：
REINHERZ, EL