Alternative Splicing of Barley Clock Genes in Response to Low Temperature.
Alternative Splicing of Barley Clock Genes in Response to Low Temperature.
复制标题
大麦时钟基因响应低温的替代剪接。
DOI：
10.1371/journal.pone.0168028
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Brown JW
中科院分区：
文献类型：
--
作者：
Calixto CP;Simpson CG;Waugh R;Brown JW
选择性剪接 (AS) 是一种从单个基因生成多个转录本的受调控机制。它广泛存在于真核生物基因组中，并提供了控制基因表达的有效方法。在低温下，AS 通过生产性 mRNA 水平的动态变化来调节拟南芥时钟基因。我们检查了大麦时钟基因中的 AS，以评估温度依赖性 AS 反应是否也发生在单子叶作物物种中。我们鉴定了各种大麦核心时钟基因的 AS 变化，包括拟南芥 AtLHY 和 AtPRR7 的大麦直系同源物，它们在响应低温时表现出最明显的 AS 变化。 AS 事件在过渡到寒冷时调节功能性和可翻译 mRNA 的水平，以及潜在的蛋白质水平。拟南芥和大麦之间存在一些 AS 事件和/或时钟基因剪接行为的保守性。此外，核心时钟基因 HvPPD-H1（光周期响应的主要决定因素和 AtPRR7 直系同源物）的新型温度依赖性 AS 在单子叶植物中是保守的。 HvPPD-H1 显示出快速、温度敏感的异构体转换，导致编码不同蛋白质异构体的 AS 变体丰度发生变化。在两个截然不同的物种中观察到的这种新型的低温控制时钟基因表达的层将有助于我们了解植物对不同环境的适应，并最终为植物改良提供一系列新的目标。
Alternative splicing (AS) is a regulated mechanism that generates multiple transcripts from individual genes. It is widespread in eukaryotic genomes and provides an effective way to control gene expression. At low temperatures, AS regulates Arabidopsis clock genes through dynamic changes in the levels of productive mRNAs. We examined AS in barley clock genes to assess whether temperature-dependent AS responses also occur in a monocotyledonous crop species. We identify changes in AS of various barley core clock genes including the barley orthologues of Arabidopsis AtLHY and AtPRR7 which showed the most pronounced AS changes in response to low temperature. The AS events modulate the levels of functional and translatable mRNAs, and potentially protein levels, upon transition to cold. There is some conservation of AS events and/or splicing behaviour of clock genes between Arabidopsis and barley. In addition, novel temperature-dependent AS of the core clock gene HvPPD-H1 (a major determinant of photoperiod response and AtPRR7 orthologue) is conserved in monocots. HvPPD-H1 showed a rapid, temperature-sensitive isoform switch which resulted in changes in abundance of AS variants encoding different protein isoforms. This novel layer of low temperature control of clock gene expression, observed in two very different species, will help our understanding of plant adaptation to different environments and ultimately offer a new range of targets for plant improvement.
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影响因子：
10.5
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Sharp PA
影响因子：
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