Pilot dietary intervention with heat-stabilized rice bran modulates stool microbiota and metabolites in healthy adults.
Pilot dietary intervention with heat-stabilized rice bran modulates stool microbiota and metabolites in healthy adults.
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DOI：
10.3390/nu7021282
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发表时间：
2015-02-16
期刊：
影响因子：
5.9
通讯作者：
Ryan EP
中科院分区：
文献类型：
--
作者：
Sheflin AM;Borresen EC;Wdowik MJ;Rao S;Brown RJ;Heuberger AL;Broeckling CD;Weir TL;Ryan EP
在动物和人体研究中，热稳定米糠（SRB）已被证明具有调节血脂和血糖，调节肠道粘膜免疫和抑制结直肠癌的作用。然而，硫酸盐还原菌对肠道微生物组成和代谢的影响以及对健康的影响在很大程度上仍然未知。开发了一项试点随机对照试验，以研究每天食用30 g SRB对粪便微生物组和代谢组的影响。7名健康参与者每天食用研究餐和零食，持续28天。在基线、干预后两周和四周，使用454焦磷酸测序和气相色谱-质谱（GC-MS）对微生物组和代谢组进行表征。在食用SRB两周和四周后，观察到八个操作分类单位（OTU）增加，包括来自双歧杆菌属和瘤胃球菌属的三个OTU（p < 0.01）。4周后，SRB组的支链脂肪酸、次级胆汁酸和其他11种假定的微生物代谢产物显著升高。最大的代谢物变化是米糠成分，吲哚-2-羧酸，这表明平均12%的增加与SRB消费。这些数据支持在成年人中进行饮食SRB干预的可行性，并支持SRB消费可以影响肠道微生物代谢。这些发现保证了未来更大的队列研究评估SRB对肠道健康的影响。
Heat-stabilized rice bran (SRB) has been shown to regulate blood lipids and glucose, modulate gut mucosal immunity and inhibit colorectal cancer in animal and human studies. However, SRB’s effects on gut microbial composition and metabolism and the resulting implications for health remain largely unknown. A pilot, randomized-controlled trial was developed to investigate the effects of eating 30 g/day SRB on the stool microbiome and metabolome. Seven healthy participants consumed a study meal and snack daily for 28 days. The microbiome and metabolome were characterized using 454 pyrosequencing and gas chromatography-mass spectrometry (GC-MS) at baseline, two and four weeks post-intervention. Increases in eight operational taxonomic units (OTUs), including three from Bifidobacterium and Ruminococcus genera, were observed after two and four weeks of SRB consumption (p < 0.01). Branched chain fatty acids, secondary bile acids and eleven other putative microbial metabolites were significantly elevated in the SRB group after four weeks. The largest metabolite change was a rice bran component, indole-2-carboxylic acid, which showed a mean 12% increase with SRB consumption. These data support the feasibility of dietary SRB intervention in adults and support that SRB consumption can affect gut microbial metabolism. These findings warrant future investigations of larger cohorts evaluating SRB’s effects on intestinal health.
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影响因子：
4.4
作者：
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DOI：
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