Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds.
Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds.
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DOI：
10.3390/nano10101913
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发表时间：
2020-09-25
期刊：
影响因子：
--
通讯作者：
Leitgeb M
中科院分区：
文献类型：
--
作者：
Hojnik Podrepšek G;Knez Ž;Leitgeb M
在这项研究中，磁性磁赤铁矿纳米粒子，这属于组的金属氧化物，功能化与壳聚糖，无毒，亲水性，生物相容性，可生物降解的生物聚合物与抗菌效果。这是使用不同的合成方法完成的，并进行了合成的壳聚糖功能化磁赤铁矿纳米粒子的性能的比较。使用扫描电子显微镜（SEM）和振动样品磁强计（VSM）进行表征。使用动态光散射（DLS）测量和激光粒度测定法进行粒度分布的表征。采用电位滴定法对不同合成方法制备的壳聚糖功能化磁赤铁矿纳米颗粒进行了表征，证实了壳聚糖功能化层的存在，为进一步固定生物活性物质提供了重要依据。此外，壳聚糖功能化磁赤铁矿纳米粒子与戊二醛（GA）或五乙烯六胺（PEHA）活化后，固定化酶胆固醇氧化酶（ChOx）和辣根过氧化物酶（HRP）的研究进行。考察了活化剂的种类和浓度、载体与酶的质量比、固定化时间和酶浓度等因素对酶固定化的影响。简而言之，使用壳聚糖悬浮交联方法（MC 2）制成的微粒被证明是最适合获得最高活性的固定化酶，和使用共价结合方法（MC 3）与壳聚糖功能化的纳米颗粒可以与MC 2竞争其应用。
In this study, magnetic maghemite nanoparticles, which belong to the group of metal oxides, were functionalized with chitosan, a non-toxic, hydrophilic, biocompatible, biodegradable biopolymer with anti-bacterial effects. This was done using different synthesis methods, and a comparison of the properties of the synthesized chitosan functionalized maghemite nanoparticles was conducted. Characterization was performed using scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM). Characterizations of size distribution were performed using dynamic light scattering (DLS) measurements and laser granulometry. A chitosan functionalization layer was confirmed using potentiometric titration on variously synthesized chitosan functionalized maghemite nanoparticles, which is important for further immobilization of bioactive compounds. Furthermore, after activation of chitosan functionalized maghemite nanoparticles with glutaraldehyde (GA) or pentaethylenehexamine (PEHA), immobilization studies of enzyme cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) were conducted. Factors influencing the immobilization of enzymes, such as type and concentration of activating reagent, mass ratio between carrier and enzyme, immobilization time and enzyme concentration, were investigated. Briefly, microparticles made using the chitosan suspension cross-linking process (MC2) proved to be the most suitable for obtaining the highest activity of immobilized enzyme, and nanoparticles functionalized with chitosan using the covalent binding method (MC3) could compete with MC2 for their applications.
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DOI：
10.1016/j.jiec.2019.11.033
发表时间：
2020-03-25
影响因子：
6.1
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