Amperometric detection in microchip electrophoresis devices: effect of electrode material and alignment on analytical performance.
Amperometric detection in microchip electrophoresis devices: effect of electrode material and alignment on analytical performance.
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DOI：
10.1002/elps.200900317
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发表时间：
2009-10
期刊：
影响因子：
2.9
通讯作者：
Lunte, Susan M.
中科院分区：
文献类型：
--
作者：
Fischer, David J.;Hulvey, Matthew K.;Regel, Anne R.;Lunte, Susan M.
关键词：
介绍了用于微芯片电泳电化学检测的不同电极材料和电极对准的制备和评价。研究了金属和碳基电极材料对灵敏度和检出限的影响。此外，直接比较了工作电极对准对分析性能的影响（在峰形、分辨率、灵敏度和LOD方面）。使用多巴胺（DA）、去甲肾上腺素（NE）和儿茶酚（CAT）作为测试分析物，发现具有末端通道排列的热解光刻胶电极的检测限最低（DA为35 nM）。除了更容易实现之外，对于所有三种分析物的检测，末端通道比对也提供了比非通道比对更好的分析性能。通道内电极对齐导致3.6倍的减少峰斜和减少峰尾的2.1倍，儿茶酚与通道末端对齐比较。
The fabrication and evaluation of different electrode materials and electrode alignments for microchip electrophoresis with electrochemical (EC) detection is described. The influences of electrode material, both metal and carbon-based, on sensitivity and limits of detection (LOD) were examined. In addition, the effects of working electrode alignment on analytical performance (in terms of peak shape, resolution, sensitivity, and LOD) were directly compared. Using dopamine (DA), norepinephrine (NE), and catechol (CAT) as test analytes, it was found that pyrolyzed photoresist electrodes with end-channel alignment yielded the lowest limit of detection (35 nM for DA). In addition to being easier to implement, end-channel alignment also offered better analytical performance than off-channel alignment for the detection of all three analytes. In-channel electrode alignment resulted in a 3.6-fold reduction in peak skew and reduced peak tailing by a factor of 2.1 for catechol in comparison to end-channel alignment.
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