Towards Tunable Protein-Carrier Wound Dressings Based on Nanocellulose Hydrogels Crosslinked with Calcium Ions.
Towards Tunable Protein-Carrier Wound Dressings Based on Nanocellulose Hydrogels Crosslinked with Calcium Ions.
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DOI：
10.3390/nano8070550
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发表时间：
2018-07-20
期刊：
影响因子：
--
通讯作者：
Ferraz N
中科院分区：
文献类型：
--
作者：
Basu A;Strømme M;Ferraz N
研究了Ca2+交联的基于木材的纳米原纤化纤维素（NFC）水凝胶，以建立关于在慢性伤口愈合背景下使用纳米纤维素用于局部药物递送应用的知识。不同大小和等电点的蛋白质以简单的浸泡程序加载到水凝胶中。监测蛋白质从水凝胶中的释放，并评估释放过程的动力学决定参数。还研究了水凝胶和蛋白质的完整性。结果表明，蛋白质和带负电荷的NFC水凝胶结构之间的静电相互作用在负载过程中起着核心作用。蛋白质的释放受Fickian扩散控制。增加的蛋白质尺寸以及正蛋白质电荷促进了从水凝胶基质的更慢和更持续的释放过程。同时，显示带正电荷的蛋白质增加后加载水凝胶强度。释放的蛋白质保持了结构稳定性和活性，因此表明Ca2+交联的NFC水凝胶可以作为治疗性蛋白质的载体而不损害蛋白质功能。可以预见，通过利用NFC水凝胶的可调电荷性质，可以定制释放曲线以满足非常特定的治疗需求。
A Ca2+-crosslinked wood-based nanofibrillated cellulose (NFC) hydrogel was investigated to build knowledge toward the use of nanocellulose for topical drug delivery applications in a chronic wound healing context. Proteins of varying size and isoelectric point were loaded into the hydrogel in a simple soaking procedure. The release of the proteins from the hydrogel was monitored and kinetics determining parameters of the release processes were assessed. The integrity of the hydrogel and proteins were also studied. The results showed that electrostatic interactions between the proteins and the negatively-charged NFC hydrogel structure played a central role in the loading process. The release of the proteins were governed by Fickian diffusion. An increased protein size, as well as a positive protein charge facilitated a slower and more sustained release process from the hydrogel matrix. At the same time, the positively-charged protein was shown to increase the post-loading hydrogel strength. Released proteins maintained structural stability and activity, thus indicating that the Ca2+-crosslinked NFC hydrogel could function as a carrier of therapeutic proteins without compromising protein function. It is foreseen that, by utilizing tunable charge properties of the NFC hydrogel, release profiles can be tailored to meet very specific treatment needs.
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影响因子：
11.2
作者：
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通讯作者：
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影响因子：
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DOI：
10.1016/j.ejpb.2013.05.015
发表时间：
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影响因子：
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影响因子：
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作者：
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