Comparative pathogenesis of an avian H5N2 and a swine H1N1 influenza virus in pigs.
Comparative pathogenesis of an avian H5N2 and a swine H1N1 influenza virus in pigs.
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DOI：
10.1371/journal.pone.0006662
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发表时间：
2009-08-17
期刊：
影响因子：
3.7
通讯作者：
Van Reeth K
中科院分区：
文献类型：
--
作者：
De Vleeschauwer A;Atanasova K;Van Borm S;van den Berg T;Rasmussen TB;Uttenthal A;Van Reeth K
猪被认为是禽流感病毒（AIV）向人类传播的中间宿主，但对猪中AIV的基本器官致病性的研究很少。我们使用了42个4周龄的流感病毒幼稚猪和两种不同的接种途径（鼻内和肠内），以比较低致病性（LP）H5 N2 AIV与H1N1猪流感病毒的发病机制。在整个感染过程中，通过滴定、免疫荧光和RT-PCR检查呼吸道和选定的呼吸道外组织的病毒复制。这两种病毒引起整个呼吸道的生产性感染，肺部的上皮细胞是主要目标。与猪病毒相比，AIV在呼吸道各级产生较低的病毒滴度和较少的抗原阳性细胞。特别是鼻粘膜的呼吸道部分仅显示出罕见的AIV阳性细胞，这与禽类与猪病毒相比鼻脱落减少有关。AIV的滴度和分布在个体猪之间变化极大，并且受到接种途径的强烈影响。与猪病毒相比，禽类的大体肺部病变和临床体征较轻，对应于肺部较低的病毒载量。脑干是发现对两种病毒的病毒和病毒RNA呈阳性的单个呼吸道外组织。我们的数据并不否定猪是AIV中间宿主的理论，但它们表明AIV需要经历遗传变化才能在猪中建立完全的复制潜力。从生物医学的角度来看，实验性LP H5 AIV感染猪可能是有用的，以检查H5疫苗或其他免疫策略提供的异源保护，以及进一步研究的分子发病机制和哺乳动物中的AIV嗜神经性。
Pigs are considered intermediate hosts for the transmission of avian influenza viruses (AIVs) to humans but the basic organ pathogenesis of AIVs in pigs has been barely studied. We have used 42 four-week-old influenza naive pigs and two different inoculation routes (intranasal and intratracheal) to compare the pathogenesis of a low pathogenic (LP) H5N2 AIV with that of an H1N1 swine influenza virus. The respiratory tract and selected extra-respiratory tissues were examined for virus replication by titration, immunofluorescence and RT-PCR throughout the course of infection. Both viruses caused a productive infection of the entire respiratory tract and epithelial cells in the lungs were the major target. Compared to the swine virus, the AIV produced lower virus titers and fewer antigen positive cells at all levels of the respiratory tract. The respiratory part of the nasal mucosa in particular showed only rare AIV positive cells and this was associated with reduced nasal shedding of the avian compared to the swine virus. The titers and distribution of the AIV varied extremely between individual pigs and were strongly affected by the route of inoculation. Gross lung lesions and clinical signs were milder with the avian than with the swine virus, corresponding with lower viral loads in the lungs. The brainstem was the single extra-respiratory tissue found positive for virus and viral RNA with both viruses. Our data do not reject the theory of the pig as an intermediate host for AIVs, but they suggest that AIVs need to undergo genetic changes to establish full replication potential in pigs. From a biomedical perspective, experimental LP H5 AIV infection of pigs may be useful to examine heterologous protection provided by H5 vaccines or other immunization strategies, as well as for further studies on the molecular pathogenesis and neurotropism of AIVs in mammals.
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影响因子：
11.8
作者：
Keawcharoen J;Oraveerakul K;Kuiken T;Fouchier RA;Amonsin A;Payungporn S;Noppornpanth S;Wattanodorn S;Theambooniers A;Tantilertcharoen R;Pattanarangsan R;Arya N;Ratanakorn P;Osterhaus DM;Poovorawan Y
通讯作者：
Poovorawan Y
影响因子：
5.5
作者：
Kyriakis, Constantinos S.;De Vleeschauwer, Annebel;Van Reeth, Kristien
通讯作者：
Van Reeth, Kristien
影响因子：
11.8
作者：
Songserm T;Amonsin A;Jam-on R;Sae-Heng N;Meemak N;Pariyothorn N;Payungporn S;Theamboonlers A;Poovorawan Y
通讯作者：
Poovorawan Y
影响因子：
9.4
作者：
Fouchier, RAM;Bestebroer, TM;Osterhaus, ADME
通讯作者：
Osterhaus, ADME
影响因子：
3.3
作者：
Ninomiya, A;Takada, A;Kida, H
通讯作者：
Kida, H