High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.
High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.
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作者：
在夏威夷短尾乌贼（Euprymna cumberopes）的定殖过程中，费氏弧菌细菌经历了从环境中的浮游运动状态到宿主粘液中的生物膜状态的生活方式转变。环二鸟苷酸（c-di-GMP）是一种细胞质信号分子，在许多细菌物种中对调节运动性生物膜转换非常重要。费氏弧菌编码50种蛋白质，预测合成和/或降解c-di-GMP，但在宿主定殖过程中c-di-GMP调节的作用尚未研究。我们研究了鱿鱼定植过程中表现出低或高水平的c-di-GMP的菌株，发现虽然低c-di-GMP菌株没有定植缺陷，但高c-di-GMP菌株严重受损。异源c-di-GMP磷酸二酯酶的表达恢复了定殖，表明该效果是由于高c-di-GMP水平。在组成性高-c-二-GMP状态下，定殖费氏弧菌表现出运动性降低，生物膜聚集体形态改变，以及一种调节相互作用，其中一种多糖位点的转录被另一种多糖的存在抑制。我们的研究结果强调了在有益动物定植过程中适当的c-di-GMP调节的重要性，说明了宿主中c-di-GMP调节的多种途径，并揭示了宿主相关聚集体中多种胞外多糖系统的相互作用。
During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus. Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species. V. fischeri encodes 50 proteins predicted to synthesize and/or degrade c-di-GMP, but a role for c-di-GMP regulation during host colonization has not been investigated. We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired. Expression of a heterologous c-di-GMP phosphodiesterase restored colonization, demonstrating that the effect is due to high c-di-GMP levels. In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide. Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.
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影响因子：
3.6
作者：
Bobrov AG;Kirillina O;Ryjenkov DA;Waters CM;Price PA;Fetherston JD;Mack D;Goldman WE;Gomelsky M;Perry RD
通讯作者：
Perry RD
影响因子：
3.6
作者：
Christensen DG;Marsden AE;Hodge-Hanson K;Essock-Burns T;Visick KL
通讯作者：
Visick KL
影响因子：
2.4
作者：
Jones, BW;Nishiguchi, MK
通讯作者：
Nishiguchi, MK
影响因子：
7.3
作者：
Hall, Cherisse L.;Lee, Vincent T.
通讯作者：
Lee, Vincent T.
影响因子：
4.4
作者：
Cohen, Joshua J.;Eichinger, Steven J.;Visick, Karen L.
通讯作者：
Visick, Karen L.