In vivo confocal microscopy detects bilateral changes of corneal immune cells and nerves in unilateral herpes zoster ophthalmicus.
In vivo confocal microscopy detects bilateral changes of corneal immune cells and nerves in unilateral herpes zoster ophthalmicus.
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DOI：
10.1016/j.jtos.2017.09.004
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Hamrah P
中科院分区：
文献类型：
--
作者：
Cavalcanti BM;Cruzat A;Sahin A;Pavan-Langston D;Samayoa E;Hamrah P
通过激光活体共聚焦显微镜（IVCM）分析单侧眼部带状疱疹（HZO）患者的双侧角膜免疫细胞和神经改变及其与角膜感觉和临床表现的相关性。这是一项前瞻性、横断面、对照单中心研究。纳入 24 名 HZO 患者的 24 只眼睛及其对侧临床未受影响的眼睛和正常对照 (n=24)。进行激光IVCM（海德堡视网膜断层扫描仪/罗斯托克角膜模块）、角膜美感测量（Cochet-Bonnet）。分析角膜树突状细胞（DC）密度和形态、基底神经纤维数量和长度的变化及其与角膜感觉、疼痛、病变位置、病程和发作次数的相关性。与对照组相比，HZO 受影响的眼睛和对侧眼睛显示中央角膜的 DC 流入显着增加（147.4±33.9、120.1±21.2 和 23.0±3.6 个细胞/mm2；p<0.0001）。与对照组（52.2±11.7 和 2.3±0.5）相比，HZO 眼中 DC 的面积（319.4±59.8 μm2；p<0.001）和树突数量（3.5±0.4 n/细胞；p=0.01）更大。 DC密度和大小与总神经长度呈中度负相关（分别为R=-0.43和R=-0.57；所有p<0.001）。特别是在疼痛患者中，神经纤维附近的神经珠和激活的 DC 出现频率更高。与对照组相比，慢性单侧 HZO 导致双侧角膜 DC 密度显着增加，角膜基底下神经减少。观察到 DC 密度和大小与神经参数呈负相关，表明免疫系统和神经系统之间存在相互作用。患有慢性疼痛的患者也表现出神经珠增加和树突状细胞活化。
To analyze bilateral corneal immune cell and nerve alterations in patients with unilateral herpes zoster ophthalmicus (HZO) by laser in vivo confocal microscopy (IVCM) and their correlation with corneal sensation and clinical findings. This is a prospective, cross-sectional, controlled single-center study. Twenty-four eyes of 24 HZO patients and their contralateral clinically unaffected eyes and normal controls (n=24) were included. Laser IVCM (Heidelberg Retina Tomograph/Rostock Cornea Module), corneal esthesiometry (Cochet-Bonnet) were performed. Changes in corneal dendritiform cell (DC) density and morphology, number and length of subbasal nerve fibers and their correlation to corneal sensation, pain, lesion location, disease duration, and number of episodes were analyzed. HZO affected and contralateral eyes showed a significant increase in DC influx of the central cornea as compared to controls (147.4±33.9, 120.1±21.2, and 23.0±3.6 cells/mm2; p<0.0001). In HZO eyes DCs were larger in area (319.4±59.8 μm2; p<0.001) and number of dendrites (3.5±0.4 n/cell; p=0.01) as compared to controls (52.2±11.7, and 2.3±0.5). DC density and size showed moderate negative correlation with total nerve length (R=−0.43 and R=−0.57, respectively; all p<0.001). A higher frequency of nerve beading and activated DCs close to nerve fibers were detected specifically in pain patients. Chronic unilateral HZO causes significant bilateral increase in corneal DC density and decrease of the corneal subbasal nerves as compared to controls. Negative correlation was observed for DC density and size to nerve parameters, suggesting interplay between the immune and nervous systems. Patients with chronic pain also showed increased nerve beading and activated DCs.
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DOI：
10.1056/nejmcp1302674
发表时间：
2013-07-18
期刊：
The New England journal of medicine
影响因子：
--
作者：
Cohen JI
通讯作者：
Cohen JI
影响因子：
--
作者：
Abendroth, Allison;Kinchington, Paul R.;Slobedman, Barry
通讯作者：
Slobedman, Barry
DOI：
10.1016/j.jtos.2016.09.004
发表时间：
2017-01
期刊：
The ocular surface
影响因子：
--
作者：
Cruzat A;Qazi Y;Hamrah P
通讯作者：
Hamrah P
影响因子：
3.4
作者：
GARCIAHIRSCHFELD, J;LOPEZBRIONES, LG;BELMONTE, C
通讯作者：
BELMONTE, C
影响因子：
5.4
作者：
Abendroth, A;Morrow, G;Slobedman, B
通讯作者：
Slobedman, B