In Vivo Confocal Microscopy of Corneal Nerves in Health and Disease.
In Vivo Confocal Microscopy of Corneal Nerves in Health and Disease.
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DOI：
10.1016/j.jtos.2016.09.004
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Hamrah P
中科院分区：
文献类型：
--
作者：
Cruzat A;Qazi Y;Hamrah P
活体共焦显微镜(IVCM)正在成为研究角膜生理和疾病不可或缺的工具。这项技术能够在细胞水平上解剖角膜结构，提供快速和非侵入性的体内角膜成像，其图像可与体外组织化学技术相媲美。由于角膜神经在调节角膜知觉、维持上皮完整性、增殖和促进伤口愈合等方面发挥着关键作用，因此对临床医生和科学家都具有重要的意义。因此，IVCM提供了一种独特的方法来研究各种情况下的角膜神经变化，例如眼部和全身疾病以及角膜手术后的角膜神经变化，而不会改变组织微环境。尤其令人感兴趣的是角膜基底下神经与其功能的相关性，这已经在正常眼、隐形眼镜佩戴者以及圆锥角膜、感染性角膜炎、角膜营养不良和神经营养性角膜病患者中进行了研究。纵向研究已经应用IVCM来研究角膜手术对神经的影响，证明了它们的再生能力。IVCM在全身性疾病的诊断和治疗中越来越重要，例如周围糖尿病神经病变，最近在眼部疾病中也是如此。在这篇综述中，我们概述了IVCM的原理和在各种眼部和全身疾病的角膜神经研究中的应用。
In vivo confocal microscopy (IVCM) is becoming an indispensable tool for studying corneal physiology and disease. Enabling the dissection of corneal architecture at a cellular level, this technique offers fast and noninvasive in vivo imaging of the cornea with images comparable to that of ex vivo histochemical techniques. Corneal nerves bear substantial relevance to clinicians and scientists alike, given their pivotal roles in regulation of corneal sensation, maintenance of epithelial integrity, and proliferation and promotion of wound healing. Thus, IVCM offers a unique method to study corneal nerve alterations in a myriad of conditions, such as ocular and systemic diseases and following corneal surgery, without altering the tissue microenvironment. Of particular interest has been the correlation of corneal subbasal nerves to their function, which has been studied in normal eyes, contact lens wearers, and patients with keratoconus, infectious keratitis, corneal dystrophies, and neurotrophic keratopathy. Longitudinal studies have applied IVCM to investigate the effects of corneal surgery on nerves, demonstrating their regenerative capacity. IVCM is increasingly important in the diagnosis and management of systemic conditions such as peripheral diabetic neuropathy and, more recently, in ocular diseases. In this review, we outline the principles and applications of IVCM in the study of corneal nerves in various ocular and systemic diseases.
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影响因子：
4.1
作者：
Al-Aqaba, Mouhamed A.;Fares, Usama;Dua, Harminder S.
通讯作者：
Dua, Harminder S.
影响因子：
4.2
作者：
Al-Aqaba, Mouhamed Ali;Otri, Ahmad Muneer;Dua, Harminder S.
通讯作者：
Dua, Harminder S.
影响因子：
4.2
作者：
Al-Aqaba, Mouhamed;Calienno, Roberta;Dua, Harminder S.
通讯作者：
Dua, Harminder S.
影响因子：
4.4
作者：
Annunziata R;Kheirkhah A;Aggarwal S;Cavalcanti BM;Hamrah P;Trucco E
通讯作者：
Trucco E
DOI：
10.1016/j.jtos.2015.01.005
发表时间：
2015-07
期刊：
The ocular surface
影响因子：
--
作者：
Aggarwal S;Kheirkhah A;Cavalcanti BM;Cruzat A;Colon C;Brown E;Borsook D;Prüss H;Hamrah P
通讯作者：
Hamrah P