DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatment.
DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatment.
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DOI：
10.1007/s10577-011-9243-2
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发表时间：
2011-11
影响因子：
2.6
通讯作者：
Hasterok, Robert
中科院分区：
文献类型：
--
作者：
Borowska, Natalia;Idziak, Dominika;Hasterok, Robert
对短柄草有丝分裂中期染色体进行 5-甲基胞嘧啶 (5-MeC) 的连续免疫定位和与染色体特异性 BAC 克隆的荧光原位杂交，以确定补体中每条染色体的特定 DNA 甲基化模式。在大多数检查的细胞中，染色体 Bd4 和 Bd5 分别具有 5S 和 35S 核糖体 DNA 的基因座，具有特征性的 5-MeC 模式。相比之下，5-MeC 沿偏着丝粒染色体对 Bd1、Bd2 和 Bd3 的分布变化更大。同源染色体之间以及染色体臂之间的甲基化位点分布存在许多差异。一些染色体位点，例如着丝粒周围区域，在所有染色体中都高度甲基化。此外，还证实了低甲基化剂 5-氮杂胞苷对二穗芽孢杆菌染色体甲基化模式的影响。研究发现，某些染色体对比其他染色体更容易发生去甲基化，但特定染色体片段的去甲基化没有明显的规律性。
Sequential immunolocalisation of 5-methylcytosine (5-MeC) and fluorescence in situ hybridisation with chromosome-specific BAC clones were performed on Brachypodium distachyon mitotic metaphase chromosomes to determine specific DNA methylation patterns of each chromosome in the complement. In the majority of cells examined, chromosomes Bd4 and Bd5, which bear the loci of 5S and 35S ribosomal DNA, respectively, had characteristic 5-MeC patterns. In contrast, the distribution of 5-MeC along the metacentric chromosome pairs Bd1, Bd2 and Bd3 was more variable. There were numerous differences in distribution of methylated sites between homologous chromosomes as well as between chromosome arms. Some chromosome sites, such as pericentromeric regions, were highly methylated in all chromosomes. Additionally, the influence of a hypomethylating agent, 5-azacytidine, on B. distachyon chromosome methylation patterns was confirmed. It was found that some chromosome pairs underwent demethylation more easily than others, but there was no apparent regularity in demethylation of particular chromosome segments.
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