Delta-aminolevulinic dehydratase is a proteasome interacting protein.
Delta-aminolevulinic dehydratase is a proteasome interacting protein.
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DOI：
10.1016/j.yexmp.2011.05.003
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发表时间：
2011-10
影响因子：
3.6
通讯作者：
French, Samuel W.
中科院分区：
文献类型：
--
作者：
Bardag-Gorce, Fawzia;French, Samuel W.
蛋白酶体与大量调节特定细胞功能的蛋白质相互作用。本研究的重点是检查蛋白酶体与 Delta 氨基乙酰丙酸脱水酶 (ALAD) 的相互作用。 ALAD 参与血红素生物合成途径，并使用多个色谱纯化步骤与 20S 蛋白酶体共同分离。 MALDI-TOF 质谱分析鉴定出该蛋白酶体共分离蛋白为 ALAD。当使用密度梯度离心分离蛋白酶体时，在 26S 蛋白酶体组分中也发现了 ALAD。它与 20S 蛋白酶体的共分离多于与 26S 蛋白酶体的共分离。此外，免疫沉淀的 ALAD 用蛋白酶体亚基抗体染色呈阳性。这些结果表明 ALAD 可能与蛋白酶体相互作用。 ALAD 可能参与调节蛋白酶体活性。当纯化的蛋白酶体与 ALAD 一起孵育时，发现 ALAD 以剂量依赖性方式改变蛋白酶体活性。这表明ALAD可能在调节蛋白酶体活性中发挥重要作用。这些数据支持这样的假设：ALAD 是血红素合成的重要酶，作为蛋白酶体相互作用蛋白也很重要。
The proteasome interacts with a large number of proteins which regulate specific cellular functions. The focus of this study is to examine the proteasome interaction with Delta aminolevulinate dehydratase (ALAD). ALAD is involved in the heme biosynthesis pathway and was co-isolated, with the 20S proteasome using several chromatographic purification steps. The MALDI-TOF mass spectrometry analysis identified this proteasome co-isolated protein as ALAD. When the proteasome was isolated using density-gradient centrifugation, ALAD was also found in the 26S proteasome fractions. It co-isolated with the 20S more than with the 26S proteasome. Furthermore, immunoprecipitated ALAD stained positive with antibodies to proteasome subunits. These results indicate that ALAD might interact with the proteasome. It is possible that ALAD is involved in modulating proteasome activity. When purified proteasomes were incubated with ALAD it was found that ALAD changes proteasome activity in a dose dependent manner. This indicates that ALAD may play a significant role in regulating proteasome activity. The data supports the hypothesis that ALAD, an important enzyme for heme synthesis, is also important as a proteasome interacting protein.
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影响因子：
3.4
作者：
Bousquet-Dubouch, Marie-Pierre;Nguen, Sheila;Bouyssie, David;Burlet-Schiltz, Odile;French, Samuel W.;Monsarrat, Bernard;Bardag-Gorce, Fawzia
通讯作者：
Bardag-Gorce, Fawzia
影响因子：
3.9
作者：
PEREIRA, ME;WILK, S
通讯作者：
WILK, S
影响因子：
7.4
作者：
Demasi, R;Penatti, CAA;Bechara, EJH
通讯作者：
Bechara, EJH
DOI：
10.1083/jcb.200204122
发表时间：
2002-07-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Molinari M;Galli C;Piccaluga V;Pieren M;Paganetti P
通讯作者：
Paganetti P
影响因子：
4.1
作者：
Bose, S;Stratford, FLL;Rivett, AJ
通讯作者：
Rivett, AJ