Near infrared spectroscopic evaluation of water in hyaline cartilage.
Near infrared spectroscopic evaluation of water in hyaline cartilage.
复制标题
DOI：
10.1007/s10439-013-0844-0
复制
发表时间：
2013-11
影响因子：
3.8
通讯作者：
Pleshko, N.
中科院分区：
文献类型：
--
作者：
Padalkar, M. V.;Spencer, R. G.;Pleshko, N.
在软骨病变的情况下，如骨关节炎，通常含水量从平均正常的60-85%增加到大于90%。由于软骨几乎没有自我修复的能力，因此需要早期检测退变的方法，而水的评估可能被证明是一种有用的诊断方法。目前的评估方法要么具有破坏性，要么耗时，要么灵敏度有限。在这里，我们研究了关节软骨的非破坏性近红外光谱（NIRS）可以用来区分自由水和结合水，并定量评估水含量的假设。以5200 cm−1和6890 cm−1为中心的吸光度分别归因于自由水和结合水的结合，以及仅归因于自由水。两个吸光度带的综合面积与组织的绝对含水量（R=0.87和R= 0.86）和百分比含水量（R=0.97和R=0.96）呈线性相关。偏最小二乘模型也被成功开发，并用于预测含水量和游离水百分比。这些数据表明，近红外光谱可用于定量测定关节软骨中的水分含量，并可能有助于在实验室环境中早期检测退行性组织变化，并可能在临床环境中进行额外的验证。
In diseased conditions of cartilage such as osteoarthritis, there is typically an increase in water content from the average normal of 60–85% to greater than 90%. As cartilage has very little capability for self-repair, methods of early detection of degeneration are required, and assessment of water could prove to be a useful diagnostic method. Current assessment methods are either destructive, time consuming or have limited sensitivity. Here, we investigated the hypotheses that non-destructive near infrared spectroscopy (NIRS) of articular cartilage can be used to differentiate between free and bound water, and to quantitatively assess water content. The absorbances centered at 5200 cm−1 and 6890 cm−1 were attributed to a combination of free and bound water, and to free water only, respectively. The integrated areas of both absorbance bands were found to correlate linearly with the absolute water content (R=0.87 and R= 0.86) and with percent water content (R=0.97 and R=0.96) of the tissue. Partial least square models were also successfully developed and were used to predict water content, and percent free water. These data demonstrate that NIRS can be utilized to quantitatively determine water content in articular cartilage, and may aid in early detection of degenerative tissue changes in a laboratory setting, and with additional validations, possibly in a clinical setting.
登录
查看更多内容
影响因子：
2.4
作者：
Bagratashvili, VN;Sobol, EN;Howdle, SM
通讯作者：
Howdle, SM
DOI：
10.1016/j.pathophys.2009.04.001
发表时间：
2010-02-01
期刊：
Pathophysiology : the official journal of the International Society for Pathophysiology
影响因子：
--
作者：
Hofmann, Gunther O;Marticke, Julia;Spahn, Gunter
通讯作者：
Spahn, Gunter
影响因子：
7
作者：
Afara, I.;Prasadam, I.;Oloyede, A.
通讯作者：
Oloyede, A.
影响因子：
3.5
作者：
Arimoto, H;Egawa, M
通讯作者：
Egawa, M
DOI：
10.1016/0731-7085(88)80013-x
发表时间：
1988-01-01
影响因子：
3.4
作者：
CACHET, T;HOOGMARTENS, J
通讯作者：
HOOGMARTENS, J