Lobe-specific proteome changes in the dorsal-lateral and ventral prostate of TRAMP mice versus wild-type mice.
Lobe-specific proteome changes in the dorsal-lateral and ventral prostate of TRAMP mice versus wild-type mice.
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DOI：
10.1002/pmic.201100008
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发表时间：
2011-06
期刊：
影响因子：
3.4
通讯作者：
Lue, Junxuan
中科院分区：
文献类型：
--
作者：
Zhang, Jinhui;Wang, Lei;Zhang, Yong;Li, Li;Higgins, LeeAnn;Lue, Junxuan
转基因小鼠前列腺腺癌（TRAMP）是前列腺癌化学预防研究中应用最广泛的转基因模型。虽然已经描述了两种叶特异性的致癌谱系，但其分子机制仍然不清楚。在这里，我们同时分析了18周的TRAMP和同窝野生型C57 BL/6小鼠的背侧（DLP）和腹侧（VP）前列腺叶的蛋白质组，通过2D-LC-MALDI-TOF/TOF与iTRAQ标记。在1%和5%的临界错误发现率下，共鉴定出483和748种蛋白质。在野生型小鼠中，发现84种蛋白质在DLP和VP之间具有不同的表达水平。在TRAMP小鼠中，118个蛋白质在TRAMP致癌过程中在DLP和/或VP中发生显著变化。其中，DLP和VP叶分别有55和36个蛋白发生了独特的变化，DLP和LP叶均有27个蛋白发生了独特的变化。免疫途径分析（IPA）能够将两个叶中发生变化的蛋白质分离到不同的途径网络中。除了作为前列腺蛋白质组学谱的参考外，我们的数据表明，在TRAMP模型中，DLP与VP中不同的蛋白质组参与了致癌作用。
The transgenic adenocarcinoma of mouse prostate (TRAMP) is the most widely used transgenic model for prostate cancer chemoprevention studies. Although two lobe-specific lineages of carcinogenesis have been described, the molecular mechanisms are still poorly defined. Here, we concurrently profiled the proteome of dorsal-lateral (DLP) and ventral (VP) prostate lobes of both TRAMP and littermate wild type C57BL/6 mice of 18 weeks by 2D-LC-MALDI-TOF/TOF with iTRAQ labeling. A total of 483 and 748 proteins were identified at critical false discovery rates of 1% and 5%. In wild-type mice, 84 proteins were found to have different expression levels between DLP and VP. In TRAMP mice, 118 proteins significantly changed in DLP and/or VP during TRAMP carcinogenesis. Among them, 55 and 36 proteins were uniquely changed in DLP or VP lobe, respectively and 27 proteins in both DLP and LP lobe. Ingenuity Pathway Analysis (IPA) was able to segregate proteins changed in two lobes into different pathway networks. In addition to serving as reference for prostate proteomic profiles, our data suggest that different sets of proteins are involved in the carcinogenesis in DLP vs. in VP in the TRAMP model.
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影响因子：
4.4
作者：
Akkina, Sanjeev K.;Zhang, Yan;Ibrahim, Hassan N.
通讯作者：
Ibrahim, Hassan N.
DOI：
10.1158/1940-6207.capr-08-0173
发表时间：
2009-05
期刊：
Cancer prevention research (Philadelphia, Pa.)
影响因子：
--
作者：
Wang L;Bonorden MJ;Li GX;Lee HJ;Hu H;Zhang Y;Liao JD;Cleary MP;Lü J
通讯作者：
Lü J
影响因子：
--
作者：
Fujimoto, Nariaki;Suzuki, Tomoharu;Kitamura, Shigeyuki
通讯作者：
Kitamura, Shigeyuki
影响因子：
4.1
作者：
Wang, J;Eltoum, IE;Lamartiniere, CA
通讯作者：
Lamartiniere, CA
影响因子：
3.3
作者：
Zhang, Jinhui;Wang, Lei;Lue, Junxuan
通讯作者：
Lue, Junxuan