Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres.
Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres.
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DOI：
10.1038/srep38910
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发表时间：
2016-12-13
影响因子：
4.6
通讯作者：
Ozawa T
中科院分区：
文献类型：
--
作者：
Yamada T;Yoshimura H;Shimada R;Hattori M;Eguchi M;Fujiwara TK;Kusumi A;Ozawa T
端粒重复序列RNA（TERRA）通过与多种端粒结合蛋白相互作用来控制端粒的结构和长度。然而，很少有人知道TERRA调节蛋白质的可及性端粒的机制，主要是因为缺乏时空信息的TERRA及其相互作用的蛋白质。我们开发了一种荧光探针来可视化内源性TERRA，以研究其在活细胞中的动力学。单粒子荧光成像显示，TERRA积累在端粒附近的区域，并捕获扩散异质核核糖核蛋白A1（hnRNPA1），从而抑制hnRNPA1定位到端粒。这些结果表明，TERRA调节hnRNPA1与端粒周围区域的端粒的结合，从而加深了对TERRA功能机制的理解。
Telomeric repeat-containing RNA (TERRA) controls the structure and length of telomeres through interactions with numerous telomere-binding proteins. However, little is known about the mechanism by which TERRA regulates the accessibility of the proteins to telomeres, mainly because of the lack of spatiotemporal information of TERRA and its-interacting proteins. We developed a fluorescent probe to visualize endogenous TERRA to investigate its dynamics in living cells. Single-particle fluorescence imaging revealed that TERRA accumulated in a telomere-neighboring region and trapped diffusive heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1), thereby inhibiting hnRNPA1 localization to the telomere. These results suggest that TERRA regulates binding of hnRNPA1 to the telomere in a region surrounding the telomere, leading to a deeper understanding of the mechanism of TERRA function.
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