Developmentally Regulated Expression of the Gene Family for Cytosolic Glutamine Synthetase in Pisum sativum.
Developmentally Regulated Expression of the Gene Family for Cytosolic Glutamine Synthetase in Pisum sativum.
复制标题
豌豆胞质谷氨酰胺合成酶基因家族的发育调控表达。
作者：
Elsbeth L. Walker;Gloria M. Coruzzi
在豌豆中，两类基因编码不同的胞质谷氨酰胺合成酶（GS）亚型。第一类包括两个几乎相同或“双”GS基因（GS 341和GS 132），而第二类包括一个单一的GS基因（GS 299）在编码区和非编码区都不同于“双”GS基因。基因特异性分析被用来监测每个基因的细胞质GS在根瘤发育过程中，在子叶萌发过程中，大量的氨必须被同化GS氮运输的两种情况下的个人贡献。对应于胞质GS的所有三个基因的mRNA显示在结节发育的时间过程中协调地积累。所有GS mRNA在由根瘤菌nifD（-）菌株形成的突变根瘤中也积累到野生型水平，表明诱导的豌豆根瘤GS表达不依赖于氨的产生。GS基因的两类不同的表达模式，观察在某些突变体根瘤和最显着的子叶发芽幼苗。细胞质GS的两类基因之间的不同模式的表达表明，他们不同的基因产物可能在豌豆发育过程中的非重叠功能。
In Pisum sativum, two classes of genes encode distinct isoforms of cytosolic glutamine synthetase (GS). The first class comprises two nearly identical or "twin" GS genes (GS341 and GS132), while the second comprises a single GS gene (GS299) distinct in both coding and noncoding regions from the "twin" GS genes. Gene-specific analyses were used to monitor the individual contribution of each gene for cytosolic GS during root nodule development and in cotyledons during germination, two contexts where large amounts of ammonia must be assimilated by GS for nitrogen transport. mRNAs corresponding to all three genes for cytosolic GS were shown to accumulate coordinately during a time course of nodule development. All the GS mRNAs also accumulate to wild-type levels in mutant nodules formed by a nifD(-) strain of Rhizobium leguminosarum indicating that induced GS expression in pea root nodules does not depend on the production of ammonia. Distinct patterns of expression for the two classes of GS genes were observed in certain mutant root nodules and most dramatically in cotyledons of germinating seedlings. The different patterns of expression between the two classes of genes for cytosolic GS suggests that their distinct gene products may serve nonoverlapping functions during pea development.
登录
查看更多内容
DOI：
10.1002/j.1460-2075.1987.tb04710.x
发表时间：
1987-01
期刊：
The EMBO Journal
影响因子：
--
作者：
S. Tingey;E. Walker;G. Coruzzi
通讯作者：
S. Tingey;E. Walker;G. Coruzzi
DOI：
--
发表时间：
1988-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Scott V. Tingey;Fong-Ying Tsai;Janice W. Edwards;Elsbeth L. Walker;Gloria M. Coruzzi
通讯作者：
Scott V. Tingey;Fong-Ying Tsai;Janice W. Edwards;Elsbeth L. Walker;Gloria M. Coruzzi
影响因子：
11.6
作者：
EDWARDS, JW;CORUZZI, GM
通讯作者：
CORUZZI, GM
DOI：
10.1073/pnas.83.6.1670
发表时间：
1986-03-01
影响因子：
11.1
作者：
CAPUT, D;BEUTLER, B;CERAMI, A
通讯作者：
CERAMI, A