Engrailed Expression during Development of a Lamprey, Lampetra japonica: A Possible Clue to Homologies between Agnathan and Gnathostome Muscles of the Mandibular Arch
Engrailed Expression during Development of a Lamprey, Lampetra japonica: A Possible Clue to Homologies between Agnathan and Gnathostome Muscles of the Mandibular Arch
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七鳃鳗 (Lampetra japonica) 发育过程中的刻痕表达：下颌弓无颌肌和颌口肌肉同源性的可能线索
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
T. Fujii
中科院分区：
文献类型：
--
作者：
N. Holland;L. Holland;Y. Honma;T. Fujii
在原肠胚阶段（6天）和最早的海鞘幼虫（31天）之间的频繁间隔固定发展七鳃鳗。用抗小鼠en蛋白的多克隆抗血清（αEnhb-1）标记七鳃鳗enrailed（en）基因，研究其表达。Western blotting显示一条主带（40± 103 Mr），可能为七鳃鳗蛋白。通过免疫组织化学和组织切片的方法，证实了en在七鳃鳗发育过程中的表达区域。在头部突出阶段（11/12天），en的表达首先在中脑/后脑边界的神经管细胞中检测到，此后不久在下颌弓的一些中胚层细胞中检测到。这些下颌弓的en表达细胞位于咽两侧肠腔起源的近轴中胚层囊泡壁中。在尾芽阶段（15天），EN表达也可检测到中胚层细胞的前唇和一些中胚层和表皮细胞的区域的尾芽。到眼斑期（18天），下颌弓中可检测到的en表达变得限于帆甲肌的细胞，该肌驱动最近形成的帆的动力冲程。在后期阶段，虽然前面的表达域消失，en的表达开始在一些表皮细胞与唇乳头，鳃裂，和鳃。我们认为七鳃鳗的velothyroideus肌肉可能是同源的硬骨鱼的某些颌骨肌肉，即腭弓提肌和鳃盖扩张肌，表达EN连续分化成肌源性中胚层可识别的肌肉类型。
Developing lampreys were fixed at frequent intervals between the gastrula stage (6 days) and the earliest ammocoete larva (31 days). Expression of lamprey engrailed (en) gene was studied by labeling with a polyclonal antiserum (αEnhb‐1) raised against mouse en protein. Western blotting of proteins from developing lampreys reveals a major band (40±103Mr), which is probably lamprey en protein. Expression domains of en were demonstrated in developing lampreys by immunohistochemistry of whole mounts and histological sections. Expression of en first becomes detectable at the head protrusion stage (11/12 days) in neural tube cells at the mid/hindbrain boundary and soon thereafter in some mesodermal cells of the mandibular arch. These en‐expressing cells of the mandibular arch are located in the walls of vesicles of paraxial mesoderm that originate by enterocoely on either side of the pharynx. At the tailbud stage (15 days), en expression is also detectable in mesodermal cells of the anterior lip and in some mesodermal and epidermal cells in the region of the tailbud. By the eye spot stage (18 days), detectable en expression in the mandibular arch becomes limited to cells of the velothyroideus muscles, which drive the power stroke of the recently formed velum. At later stages, while the preceding expression domains fade, en expression begins in some epidermal cells associated with the lip papillae, gill slits, and nostril. We suggest that the velothyroideus muscles of lampreys may be homologous to certain jaw muscles of teleosts–namely, the levator arcus palatini and the dilator operculi, which express en continuously while differentiating from the myogenic mesoderm into identifiable muscle types.
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斑马鱼中 engrailed 样抗原的表达多样性。
DOI：
10.1242/dev.112.3.821
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hatta,K;Bremiller,R;Westerfield,M;Kimmel,CB
通讯作者：
Kimmel,CB
Lbx1 表达和青蛙肢体发育。
影响因子：
2.4
作者：
Sabo,MichelleC;Nath,Kimberly;Elinson,RichardP
通讯作者：
Elinson,RichardP
影响因子：
4.6
作者：
E. Davidson
通讯作者：
E. Davidson
影响因子：
56.9
作者：
JOYNER, AL;HERRUP, K;ROSSANT, J
通讯作者：
ROSSANT, J
环节动物早期发育和神经发生过程中 engrailed 类基因的片段表达。
DOI：
10.1242/dev.113.3.805
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Wedeen,CJ;Weisblat,DA
通讯作者：
Weisblat,DA