Fentanyl Induces Cerebellar Internal Granular Cell Layer Apoptosis in Healthy Newborn Pigs.
Fentanyl Induces Cerebellar Internal Granular Cell Layer Apoptosis in Healthy Newborn Pigs.
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DOI：
10.3389/fneur.2018.00294
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发表时间：
2018
影响因子：
3.4
通讯作者：
Thoresen M
中科院分区：
文献类型：
--
作者：
Sabir H;Dingley J;Scull-Brown E;Chakkarapani E;Thoresen M
像芬太尼这样的阿片类药物经常用于新生儿的镇痛和镇静。到目前为止，据报道它们是安全的，符合使用条件。小脑在过去几年里已经成为神经发育研究的焦点，因为它在长期的运动、认知和其他行为变化中起着重要作用。小脑皮层在小脑的协调作用中起着重要作用，极易受到损伤和生长障碍的影响。本研究旨在探讨芬太尼静脉注射对健康新生猪小脑细胞凋亡的影响。13头健康猪（中位年龄小于12 h）随机分为(1)正常体温下静脉注射24 h芬太尼（NTFe, n = 6）和(2)正常体温下不通气的对照组（NTCTR, n = 7）。苏木精-伊红染色后对小脑切片进行形态学评价。此外，对成对切片进行细胞死亡免疫染色[Cleaved caspase-3和末端脱氧核苷酸转移酶介导的脱氧尿苷-三磷酸镍端标记（TUNEL）]，并在内部颗粒细胞层的特定区域计数阳性细胞。共计数3个小脑回细胞。我们发现，NTFe组内颗粒细胞层的凋亡形态细胞增加。为了量化，我们发现(1)组的细胞死亡显著增加[caspase-3阳性细胞组(1)8（1 - 22）比(2)1（1 - 6）和tunel阳性细胞(1)6（1 - 10）比(2)1（0-4）的中位数（范围）数目]。在两组中，浦肯野细胞的数量没有差异。两组在24 h内的生理参数比较稳定。连续24小时静脉输注芬太尼可增加健康新生猪小脑内颗粒细胞层凋亡。
Opioids like fentanyl are regularly used in neonates for analgesia and sedation. So far, they have been reported to be safe and eligible to use. The cerebellum has become a focus of neurodevelopmental research within the last years, as it is known to play an important role in long-lasting motor, cognitive, and other behavioral changes. The cerebellar cortex is of major importance in the coordinative role of the cerebellum and highly vulnerable to injury and impaired growth. This study was performed to evaluate the apoptotic effect of intravenous fentanyl infusion on the cerebellum in healthy newborn pigs. Thirteen healthy pigs (<median 12 h old) were randomized into (1) 24 h of intravenous fentanyl at normothermia (NTFe, n = 6) or (2) non-ventilated controls at normothermia (NTCTR, n = 7). Cerebellar sections were morphologically assessed after staining with hematoxylin–eosin. In addition, paired sections were immuno-stained for cell death [Cleaved caspase-3 and terminal deoxynucleotidyl transferase-mediated deoxyuridine-triphosphate nick-end labeling (TUNEL)], and positive cells were counted in defined areas of the internal granular cell layer. In total, cells in three cerebellar gyri were counted. We found that there was an increase in cells with apoptotic morphology in the internal granular cell layer in the NTFe group. For quantification, we found a significant increase in cell death in group (1) [median (range) number of caspase-3-positive cell group (1) 8 (1–22) vs. group (2) 1 (1–6) and TUNEL-positive cells (1) 6 (1–10) vs. (2) 1 (0–4)]. In both groups, there was no difference in the number of Purkinje cells. Both groups had comparable and stable physiological parameters throughout the 24 h period. Twenty-four hours of continuous intravenous fentanyl infusion increased apoptosis in the internal granular cell layer in the cerebellum of healthy newborn pigs.
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影响因子：
6.1
作者：
Biran, Valerie;Heine, Vivi M.;Verney, Catherine;Sheldon, R. Ann;Spadafora, Ruggero;Vexler, Zinaida S.;Rowitch, David H.;Ferriero, Donna M.
通讯作者：
Ferriero, Donna M.
影响因子：
5.3
作者：
HAUSER, KF;GURWELL, JA;TURBEK, CS
通讯作者：
TURBEK, CS
影响因子：
2.5
作者：
DOBBING, J;SANDS, J
通讯作者：
SANDS, J
DOI：
10.1001/archpediatrics.2011.43
发表时间：
2011-08-01
影响因子：
--
作者：
Jacobs, Susan E.;Morley, Colin J.;Doyle, Lex W.
通讯作者：
Doyle, Lex W.
影响因子：
38.9
作者：
Chakkarapani, Elavazhagan;Thoresen, Marianne;Dingley, John
通讯作者：
Dingley, John