Annulus fibrosus cell characteristics are a potential source of intervertebral disc pathogenesis.
Annulus fibrosus cell characteristics are a potential source of intervertebral disc pathogenesis.
复制标题
DOI：
10.1371/journal.pone.0096519
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Jin L;Liu Q;Scott P;Zhang D;Shen F;Balian G;Li X
在椎间盘退变的终末期，软骨、骨、内皮细胞和神经元出现与恶化的状况相关。异常细胞的起源尚不清楚。本研究使用一种体外模型和两种体内模型研究纤维环（AF）中祖细胞的特性。软骨形成培养基培养兔AF细胞，胶原和聚集蛋白聚糖的表达显着增加。在暴露于成骨条件下，培养物显示出增加的矿化和骨桥蛋白，runx 2和bmp 2基因的表达。在体内皮下植入实验中使用了两种模型：1）脱矿骨基质（DBM）圆柱体中的兔AF组织（DBM/AF），以及2）大鼠完整和针刺腰椎间盘。DBM/AF植入裸鼠体内1个月后，检测到AF组织中的骨形成，并存在肥大的软骨细胞和成骨细胞。除了胶原I和II，免疫染色显示植入后4个月DBM/AF标本中的胶原X和骨钙素表达。在损伤的椎间盘中检测到类似的变化。6个月时，几乎整个针刺椎间盘已骨化。结果表明，AF细胞具有祖细胞的特性，在适当的刺激下，能够在体外和体内分化为软骨细胞和成骨细胞。重要的是，这些细胞可能是椎间盘退变生物治疗的靶点。
In the end stage of intervertebral disc degeneration, cartilage, bone, endothelial cells, and neurons appear in association with the worsening condition. The origin of the abnormal cells is not clear. This study investigated the properties of progenitor cells in the annulus fibrosus (AF) using one in vitro and two in vivo models. Cultivation of rabbit AF cells with chondrogenic media significantly increased expressions of collagen and aggrecan. Upon exposure to osteogenic conditions, the cultures showed increased mineralization and expression of osteopontin, runx2, and bmp2 genes. Two models were used in the in vivo subcutaneous implantation experiments: 1) rabbit AF tissue in a demineralized bone matrix (DBM) cylinder (DBM/AF), and, 2) rat intact and needle punctured lumbar discs. Bone formation in the AF tissue was detected and hypertrophic chondrocytes and osteoblasts were present 1 month after implantation of the DBM/AF to nude mice. In addition to collagen I and II, immunostaining shows collagen X and osteocalcin expression in DBM/AF specimens 4 months after implantation. Similar changes were detected in the injured discs. Almost the entire needle punctured disc had ossified at 6 months. The results suggest that AF cells have characteristics of progenitor cells and, under appropriate stimuli, are capable of differentiating into chondrocytes and osteoblasts in vitro as well as in vivo. Importantly, these cells may be a target for biological treatment of disc degeneration.
登录
查看更多内容
影响因子：
4.5
作者：
Cui, Min;Wan, Yuqing;Li, Xudong
通讯作者：
Li, Xudong
影响因子：
5.3
作者：
Feng, Gang;Yang, Xinlin;Li, Xudong
通讯作者：
Li, Xudong
影响因子：
168.9
作者：
Freemont, AJ;Peacock, TE;Jayson, MIV
通讯作者：
Jayson, MIV
影响因子：
15.9
作者：
Antoniou, J;Steffen, T;Alini, M
通讯作者：
Alini, M
影响因子：
8.2
作者：
Jin L;Wan Y;Shimer AL;Shen FH;Li XJ
通讯作者：
Li XJ