MBD-isolated Genome Sequencing provides a high-throughput and comprehensive survey of DNA methylation in the human genome.
MBD-isolated Genome Sequencing provides a high-throughput and comprehensive survey of DNA methylation in the human genome.
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MBD分离的基因组测序提供了对人基因组中DNA甲基化的高通量和全面调查。
DOI：
10.1093/nar/gkp992
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发表时间：
2010-01
影响因子：
14.9
通讯作者：
Ting AH
中科院分区：
文献类型：
--
作者：
Serre D;Lee BH;Ting AH
DNA甲基化是一种表观遗传修饰，通过调节基因表达和维持基因组结构参与正常发育过程和疾病状态。传统的DNA甲基化分析方法，如亚硫酸氢盐测序、甲基化敏感的限制性内切酶消化和基于阵列的检测技术，具有阻碍高通量全基因组分析的主要局限性。我们描述了一种新的技术，MBD分离的基因组测序（MiGS），它结合了沉淀的甲基化DNA的重组甲基-CpG结合结构域的MBD 2蛋白和测序的分离的DNA的大规模并行测序仪。我们利用MiGS研究了三个具有不同程度DNA甲基化的同基因癌细胞系。我们成功地检测到这些细胞中以前已知的甲基化区域，并确定了数百个新的甲基化区域。该技术具有高度特异性和灵敏度，可应用于任何生物学环境，以在基因组规模上鉴定差异甲基化区域。
DNA methylation is an epigenetic modification involved in both normal developmental processes and disease states through the modulation of gene expression and the maintenance of genomic organization. Conventional methods of DNA methylation analysis, such as bisulfite sequencing, methylation sensitive restriction enzyme digestion and array-based detection techniques, have major limitations that impede high-throughput genome-wide analysis. We describe a novel technique, MBD-isolated Genome Sequencing (MiGS), which combines precipitation of methylated DNA by recombinant methyl-CpG binding domain of MBD2 protein and sequencing of the isolated DNA by a massively parallel sequencer. We utilized MiGS to study three isogenic cancer cell lines with varying degrees of DNA methylation. We successfully detected previously known methylated regions in these cells and identified hundreds of novel methylated regions. This technique is highly specific and sensitive and can be applied to any biological settings to identify differentially methylated regions at the genomic scale.
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DOI：
10.1007/978-1-59745-522-0_6
发表时间：
2009
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Rauch, Tibor A.;Pfeifer, Gerd P.
通讯作者：
Pfeifer, Gerd P.
影响因子：
64.8
作者：
GRUENBAUM, Y;CEDAR, H;RAZIN, A
通讯作者：
RAZIN, A
DOI：
10.1002/ssu.2980030304
发表时间：
1987-01-01
期刊：
SEMINARS IN SURGICAL ONCOLOGY
影响因子：
--
作者：
FEINBERG, AP;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
4.5
作者：
Schuebel KE;Chen W;Cope L;Glöckner SC;Suzuki H;Yi JM;Chan TA;Van Neste L;Van Criekinge W;van den Bosch S;van Engeland M;Ting AH;Jair K;Yu W;Toyota M;Imai K;Ahuja N;Herman JG;Baylin SB
通讯作者：
Baylin SB
影响因子：
8
作者：
Rice, JC;Massey-Brown, KS;Futscher, BW
通讯作者：
Futscher, BW