Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.
Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.
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DOI：
10.1038/s41467-018-03551-y
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发表时间：
2018-03-28
影响因子：
16.6
通讯作者：
Foster SJ
中科院分区：
文献类型：
--
作者：
Turner RD;Mesnage S;Hobbs JK;Foster SJ
生物聚合物复合细胞壁保持细胞形状并抵抗植物，真菌和细菌的力量。肽聚糖是一种重要的抗生素靶点和免疫调节剂，在细菌中发挥这种作用。肽聚糖的教科书结构模型是高度有序的结晶材料。在这里，我们使用原子力显微镜（AFM）图像的肽聚糖从大肠杆菌中前所未有的细节个别聚糖链。我们量化和映射的程度链在一个类似的方向（取向顺序），显示它是比以前描绘的有序得多。结合原子力显微镜与尺寸排阻色谱法，我们揭示了聚糖链长达200 nm。我们发现，改变细胞形状与肽聚糖的生物物理性质的实质性变化。E.正常杆状的大肠杆菌是长的，并且是圆周方向的，但是当球形被诱导（化学或遗传）时，聚糖变得短而无序。细菌细胞壁中肽聚糖的分子结构仍不清楚。在这里，Turner等人使用原子力显微镜以前所未有的细节对肽聚糖中的单个聚糖链进行成像，揭示了其分子组织的新特征。
Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria. Peptidoglycan, a crucial antibiotic target and immunomodulator, performs this role in bacteria. The textbook structural model of peptidoglycan is a highly ordered, crystalline material. Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail. We quantify and map the extent to which chains are oriented in a similar direction (orientational order), showing it is much less ordered than previously depicted. Combining AFM with size exclusion chromatography, we reveal glycan chains up to 200 nm long. We show that altered cell shape is associated with substantial changes in peptidoglycan biophysical properties. Glycans from E. coli in its normal rod shape are long and circumferentially oriented, but when a spheroid shape is induced (chemically or genetically) glycans become short and disordered. The molecular architecture of peptidoglycan in the bacterial cell wall remains unclear. Here, Turner et al. use atomic force microscopy to image individual glycan strands in peptidoglycan at an unprecedented detail, revealing novel features of its molecular organisation.
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影响因子：
3.6
作者：
Turner RD;Vollmer W;Foster SJ
通讯作者：
Foster SJ
影响因子：
64.5
作者：
Paradis-Bleau C;Markovski M;Uehara T;Lupoli TJ;Walker S;Kahne DE;Bernhardt TG
通讯作者：
Bernhardt TG
影响因子：
11.4
作者：
Kruse, T;Moller-Jensen, J;Gerdes, K
通讯作者：
Gerdes, K
DOI：
10.1073/pnas.0805309105
发表时间：
2008-12-09
影响因子：
11.1
作者：
Huang, Kerwyn Casey;Mukhopadhyay, Ranjan;Wingreen, Ned S.
通讯作者：
Wingreen, Ned S.
影响因子：
3.2
作者：
Takacs, Constantin N.;Poggio, Sebastian;Jacobs-Wagner, Christine
通讯作者：
Jacobs-Wagner, Christine