Localized alteration of microtubule polymerization in response to guidance cues.
Localized alteration of microtubule polymerization in response to guidance cues.
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DOI：
10.1002/jnr.22478
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发表时间：
2010-11-01
影响因子：
4.2
通讯作者：
Geller, Herbert M.
中科院分区：
文献类型：
--
作者：
Kelly, Terri-Ann N.;Katagiri, Yasuhiro;Vartanian, Keri B.;Kumar, Pramukta;Chen, Inn-Inn;Rosoff, William J.;Urbach, Jeffery S.;Geller, Herbert M.
抑制微管动态不稳定性防止生长锥转向响应于指导线索，但具体的变化，微管聚合生长锥遇到的边界还没有被调查。在这项研究中，我们研究了微管聚合的速度和方向响应于可溶性神经生长因子（NGF）和固定化硫酸软骨素蛋白聚糖（CSPGs）表达增强GFPEB 3在大鼠嗜铬细胞瘤（PC 12）细胞。使用延时落射荧光显微镜在活细胞中监测GFP-EB 3彗星。使用自动跟踪系统，将微管聚合速率计算为EB 3彗星的帧到帧位移。我们的研究结果表明，微管聚合率增加神经生长因子治疗后，而与CSPGs接触降低微管聚合率。微管聚合速率的这种降低特异性地定位于与CSPG直接接触的神经突，而不是非接触性神经突。此外，我们发现在CSPG边界的神经突中逆行方向聚合的微管的百分比增加，同时逆行微管聚合的速率降低。这些结果表明，微管动力学的局部变化是生长锥对指导线索反应的重要组成部分。
Inhibition of microtubule dynamic instability prevents growth cone turning in response to guidance cues, yet specific changes in microtubule polymerization as growth cones encounter boundaries have not been investigated. In this study, we examined the rate and direction of microtubule polymerization in response to soluble nerve growth factor (NGF) and immobilized chondroitin sulfate proteoglycans (CSPGs) by expressing enhanced GFPEB3 in rat pheochromocytoma (PC12) cells. GFP-EB3 comets were monitored in live cells using time-lapse epifluorescent microscopy. Using an automated tracking system, the rate of microtubule polymerization was calculated as the frame-to-frame displacement of EB3 comets. Our results demonstrate that the rate of microtubule polymerization is increased following NGF treatment, while contact with CSPGs decreases microtubule polymerization rates. This reduction in microtubule polymerization rates was specifically localized to neurites in direct contact with CSPGs, and not at non-contacting neurites. Additionally, we found an increase in the percentage of microtubules polymerizing in the retrograde direction in neurites at CSPG boundaries with a concomitant decrease in the rate of retrograde microtubule polymerization. These results implicate localized changes in microtubule dynamics as an important component of the growth cone response to guidance cues.
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影响因子：
3.4
作者：
KATOHSEMBA, R;MATSUDA, M;OOHIRA, A
通讯作者：
OOHIRA, A
影响因子：
4.2
作者：
Lord-Fontaine, Stephanie;Yang, Fan;McKerracher, Lisa
通讯作者：
McKerracher, Lisa
影响因子：
5.3
作者：
Dent, EW;Callaway, JL;Kalil, K
通讯作者：
Kalil, K
影响因子：
3
作者：
Lee, Aih Cheun;Suter, Daniel M.
通讯作者：
Suter, Daniel M.
神经生长因子促进神经突生长期间微管组成和稳定性的调节。
DOI：
10.1083/jcb.103.2.545
发表时间：
1986-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Black MM;Aletta JM;Greene LA
通讯作者：
Greene LA