Regulation of microtubule composition and stability during nerve growth factor-promoted neurite outgrowth.
Regulation of microtubule composition and stability during nerve growth factor-promoted neurite outgrowth.
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神经生长因子促进神经突生长期间微管组成和稳定性的调节。
DOI：
10.1083/jcb.103.2.545
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发表时间：
1986-08
期刊：
影响因子：
--
通讯作者：
Greene LA
中科院分区：
文献类型：
--
作者：
Black MM;Aletta JM;Greene LA
我们已经使用了神经生长因子（NGF）的PC 12嗜铬细胞瘤细胞作为一个模型系统，研究微管专业化与轴突生长。用NGF处理的PC 12细胞停止增殖并延伸神经突。长期的NGF治疗导致PC 12细胞中聚合的总细胞微管蛋白的比例增加2 - 3倍。在此参数的增加首先变得明显，在2-4天与NGF和稳步增加之后。PC 12细胞的微管相关蛋白（MAPs）在暴露于NGF后也发生了一些变化。在免疫沉淀试验中，我们观察到用NGF处理后MAP-2的水平至少增加了几倍。我们还发现，与表观Mr为64 K，67 K和80 K的三个MAP类的组成被NGF处理改变。这些MAP，最近命名为“chartins”，在生物化学和免疫学上不同于类似大小的tau MAP（Peng et al.，1985 Brain Res. 361：200; Meclantz和所罗门，1985 Proc. Natl. Acad. Sci. 82：6581）。在二维等电聚焦x SDS聚丙烯酰胺凝胶中，每个图蛋白MAP类解析为一组具有相似表观Mr但不同pI的蛋白质。肽图谱分析证实，组成每个chartin MAP类的等电变体在一级结构中密切相关。几个显着的差异，在与或无NGF生长的PC 12细胞的chartin地图的组成一致观察。特别是，长期的神经生长因子治疗后，丰度的更酸性的变体的每个chartin MAP类显着增强相对于更基本的成员。这种情况发生时，相对于总细胞蛋白，每个MAP类的丰度总体上没有实质性变化。结合体内磷酸化和肽图谱实验的结果表明，神经生长因子诱导的查廷MAP物种不是主要的翻译产物，但产生postperfectationally，显然是由其他查廷MAP的差异磷酸化。这些观察结果表明，神经生长因子治疗的PC 12细胞导致的变化，在翻译后处理的查廷地图。这些变化的时间过程非常类似于聚合的细胞微管蛋白的比例增加和神经突生长。神经突生长和稳定的重要事件之一似乎是从细胞体延伸到神经突尖端的微管束的形成。（400字处截断摘要）
We have used the nerve growth factor (NGF)-responsive line of PC12 pheochromocytoma cells as a model system to study microtubule specializations associated with neurite outgrowth. PC12 cells treated with NGF cease proliferating and extend neurites. Long-term NGF treatment results in a two- to threefold increase in the proportion of total cellular tubulin that is polymerized in PC12 cells. The increase in this parameter first becomes apparent at 2-4 d with NGF and increases steadily thereafter. Several changes in microtubule- associated proteins (MAPs) of PC12 cells also occur after exposure to NGF. In immunoprecipitation assays, we observed the levels of MAP-2 to increase by at least several-fold after treatment with NGF. We also found that the compositions of three MAP classes with apparent Mr of 64K, 67K, and 80K are altered by NGF treatment. These MAPs, recently designated "chartins," are biochemically and immunologically distinct from the similarly-sized tau MAPs (Peng et al., 1985 Brain Res. 361: 200; Magendantz and Solomon, 1985 Proc. Natl. Acad. Sci. 82: 6581). In two-dimensional isoelectric focusing x SDS polyacrylamide gels, each chartin MAP class resolves into a set of proteins of similar apparent Mr but distinct pI. Peptide mapping analyses confirm that the isoelectric variants comprising each chartin MAP class are closely related in primary structure. Several striking differences in the composition of the chartin MAPs of PC12 cells grown with or without NGF were consistently observed. In particular, following longterm NGF treatment, the abundances of the more acidic variants of each chartin MAP class were markedly enhanced relative to the more basic members. This occurs without substantial changes in the abundance of each MAP class as a whole relative to total cell protein. The combined results of in vivo phosphorylation and peptide mapping experiments indicate that the NGF-inducible chartin MAP species are not primary translation products, but are generated posttranslationally, apparently by differential phosphorylation of other chartin MAPs. These observations suggest that NGF treatment of PC12 cells leads to changes in the posttranslational processing of the chartin MAPs. The time course of these changes closely resembles that for the increase in the proportion of cellular tubulin that is polymerized and for neurite outgrowth. One of the important events in the growth and stabilization of neurites appears to be the formation of microtubule bundles that extend from the cell body to the tips of the neurites.(ABSTRACT TRUNCATED AT 400 WORDS)
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DOI：
10.1083/jcb.101.4.1371
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Binder LI;Frankfurter A;Rebhun LI
通讯作者：
Rebhun LI
DOI：
10.1111/j.1432-1033.1977.tb11726.x
发表时间：
1977-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
FELLOUS, A;FRANCON, J;NUNEZ, J
通讯作者：
NUNEZ, J
DOI：
10.1073/pnas.82.16.5404
发表时间：
1985-01-01
影响因子：
11.1
作者：
BLOOM, GS;LUCA, FC;VALLEE, RB
通讯作者：
VALLEE, RB
影响因子：
64.5
作者：
DUERR, A;PALLAS, D;SOLOMON, F
通讯作者：
SOLOMON, F
影响因子：
2.9
作者：
BLACK, MM;COCHRAN, JM;KURDYLA, JT
通讯作者：
KURDYLA, JT