Influence of Staphylococcus epidermidis biofilm on the mechanical strength of soft tissue allograft.
Influence of Staphylococcus epidermidis biofilm on the mechanical strength of soft tissue allograft.
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DOI：
10.1002/jor.25360
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发表时间：
2023-02
影响因子：
2.8
通讯作者：
Flanigan, David C.
中科院分区：
文献类型：
--
作者：
Sorensen, Hanna H.;Magnussen, Robert A.;DiBartola, Alex C.;Mallory, Noah T.;Litsky, Alan S.;Stoodley, Paul;Swinehart, Steven D.;Duerr, Robert A.;Kaeding, Christopher C.;Flanigan, David C.
我们试图确定细菌接种和暴露时间对软组织肌腱移植物机械完整性的影响。将表皮葡萄球菌培养物接种于人尸体胫骨后肌腱上以生长生物膜。将10%生长培养基中的低接种物孵育30分钟以复制临床感染的条件。评估的生长条件包括100、1000、10，000菌落形成单位（CFU）的接种物浓度。使用MTS Bionix系统进行测试，以评估细菌生物膜对肌腱强度的影响。对钢筋束进行了失效载荷试验，并根据最大力和横截面积获得极限拉伸强度。将肌腱起点至最大位移的位移归一化为肌腱长度，以获得应变值。计算了筋的力-位移和应力-应变关系，并确定了杨氏模量。弹性模量和极限拉伸强度随生物负载的增加而降低。与接种10，000 CFU的肌腱相比，未接种对照组的杨氏模量更大（p = 0.0011），但不受100和1000 CFU细菌浓度的影响（p = 0.054，p = 0.078）。生物负载增加与失效峰值负载降低相关（p = 0.043），但与10，000和1000 CFU生长条件下的对照品相比最为显著（p = 0.0005，p = 0.049）。S. epidermidis增加了人尸体肌腱的弹性并降低了极限拉伸应力，随着生物负载的增加，效果也越来越明显。了解ACLR后亚临床和慢性感染对机械强度的影响可能会减少移植物失败并延长重建的完整性。
We sought to determine the impact of bacterial inoculation and length of exposure on the mechanical integrity of soft tissue tendon grafts. Cultures of Staphylococcus epidermidis were inoculated on human tibialis posterior cadaveric tendon to grow biofilms. A low inoculum in 10% growth medium was incubated for 30 min to replicate conditions of clinical infection. Growth conditions assessed included inoculum concentrations of 100, 1000, 10,000 colony‐forming units (CFUs). Tests using the MTS Bionix system were performed to assess the influence of bacterial biofilms on tendon strength. Load‐to‐failure testing was performed on the tendons, and the ultimate tensile strength was obtained from the maximal force and the cross‐sectional area. Displacements of tendon origin to maximal displacement were normalized to tendon length to obtain strain values. Tendon force‐displacement and stress‐strain relationships were calculated, and Young's modulus was determined. Elastic modulus and ultimate tensile strength decreased with increasing bioburden. Young's modulus was greater in uninoculated controls compared to tendons inoculated at 10,000 CFU (p = 0.0011) but unaffected by bacterial concentrations of 100 and 1000 CFU (p = 0.054, p = 0.078). Increasing bioburden was associated with decreased peak load to failure (p = 0.043) but was most significant compared to the control under the 10,000 and 1000 CFU growth conditions (p = 0.0005, p = 0.049). The presence of S. epidermidis increased elasticity and decreased ultimate tensile stress of human cadaveric tendons, with increasing effect noted with increasing bioburden. Understanding the impact of subclinical and chronic infection on mechanical strength following ACLR may reduce graft failure and prolong the integrity of the reconstruction.
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影响因子：
3.3
作者：
Herzog MM;Marshall SW;Lund JL;Pate V;Mack CD;Spang JT
通讯作者：
Spang JT
影响因子：
3.1
作者：
Perez, Kimberly;Patel, Robin
通讯作者：
Patel, Robin
影响因子：
3.8
作者：
Condello, V.;Zdanowicz, U.;Kon, E.
通讯作者：
Kon, E.
影响因子：
3.8
作者：
Offerhaus, Christoph;Balke, Maurice;Hoeher, Juergen
通讯作者：
Hoeher, Juergen
影响因子：
1.9
作者：
Hantes, Michael E.;Raoulis, Vasilios A.;Malizos, Konstantinos N.
通讯作者：
Malizos, Konstantinos N.