Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels.
Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels.
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通过巢式实时 PCR 检测红松鼠 (Sciurus vulgaris) 和灰松鼠 (Sciurus carolinensis) 中的松鼠痘病毒。
DOI：
10.1186/1746-6148-6-33
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发表时间：
2010-06-08
影响因子：
2.6
通讯作者：
Chantrey, Julian
中科院分区：
文献类型：
--
作者：
Atkin, Janus W.;Radford, Alan D.;Coyne, Karen P.;Stavisky, Jenny;Chantrey, Julian
松鼠痘病毒（SQPV）对红松鼠（Sciurus vulgaris）具有高致病性，并且是英格兰和威尔士大部分地区物种局部灭绝的重要因素，其中感染在东部灰松鼠（Sciurus carolinensis）种群中是地方性的。虽然巢式PCR检测已成功用于研究SQPV的流行病学，但样品处理时间长，且检测无法定量。该项目描述了SQPV实时PCR的设计和优化。与巢式PCR的比较表明，实时检测灵敏度高出一个对数，并且能够检测每mg组织约144个基因组拷贝。实时PCR已被用来量化组织中的病毒基因组载量从患病的和明显健康的红松鼠和灰松鼠，并表明，病毒的滴度从患病的红松鼠组织中是相当高的，甚至发现在灰松鼠皮肤病变。
Squirrel poxvirus (SQPV) is highly pathogenic to red squirrels (Sciurus vulgaris), and is a significant contributing factor to the local extinction of the species in most parts of England and Wales, where infection is endemic in Eastern grey squirrel (Sciurus carolinensis) populations. Although a nested PCR assay has been used successfully to study the epidemiology of SQPV, samples have a long processing time and the assay is not quantifiable. This project describes the design and optimization of a real-time PCR for SQPV. Comparison with the nested PCR showed the real-time assay to be more sensitive by one log and able to detect approximately 144 genome copies per mg of tissue. The real-time PCR has been used to quantify viral genome load in tissues from diseased and apparently healthy red and grey squirrels, and suggests that the titre of virus in tissues from diseased red squirrels is considerably higher than that found even in a grey squirrel with cutaneous lesions.
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影响因子：
2.2
作者：
SAINSBURY, AW;GURNELL, J
通讯作者：
GURNELL, J
影响因子：
9.3
作者：
Scaramozzino, Natale;Ferrier-Rembert, Audrey;Garin, Daniel
通讯作者：
Garin, Daniel
影响因子：
0.8
作者：
YERUHAM, I;ABRAHAM, A;NYSKA, A
通讯作者：
NYSKA, A
影响因子：
4.2
作者：
Rushton, S. P.;Lurz, P. W. W.;Sainsbury, A. W.
通讯作者：
Sainsbury, A. W.
DOI：
10.1098/rspb.1993.0028
发表时间：
1993-03-22
影响因子：
4.7
作者：
KENWARD, RE;HOLM, JL
通讯作者：
HOLM, JL