A mycobacterial DivIVA domain-containing protein involved in cell length and septation.
A mycobacterial DivIVA domain-containing protein involved in cell length and septation.
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DOI：
10.1099/mic.0.000952
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Bhatt A
中科院分区：
文献类型：
--
作者：
Pickford H;Alcock E;Singh A;Kelemen G;Bhatt A
分枝杆菌细胞通过细胞壁物质的极性沉积而伸长，类似于丝状链霉菌，其含有尖端组织中心。卷曲螺旋蛋白如DivIVA在这一过程中发挥重要作用。结核分枝杆菌（Mycobacterium tuberculosis）是结核病的病原体，其基因组编码许多卷曲螺旋蛋白，这些卷曲螺旋蛋白与DivIVA同源，在分枝杆菌细胞伸长中具有潜在作用。在这里，我们描述了对耻垢分枝杆菌MSMEG_2416的研究。结核Rv2927c.之前的两项独立研究表明，MSMEG_2416参与了分隔（随后称为sepIVA）。与这些先前的报道相反，我们发现sepIVA通过产生可行的无效突变体而在实验室培养基中生长。然而，突变菌株确实显示出许多差异，包括菌落形态和生物膜形成的变化，其可以在与sepIVA以及Rv2927c（来自M.结核然而，细胞壁脂质的分析没有发现突变株的脂质谱的任何改变。突变体的显微镜检查显示细胞较长，具有更多的隔膜，其以不规则的间隔发生，通常产生小隔室，与先前条件性耗竭后研究中观察到的特征相似，突出了sepIVA在分枝杆菌生长中的作用。
Mycobacterial cells elongate via polar deposition of cell wall material, similar to the filamentous Streptomyces species, which contain a tip-organizing centre. Coiled-coiled proteins such as DivIVA play an important role in this process. The genome of Mycobacterium tuberculosis , the causative agent of tuberculosis, encodes many coiled-coil proteins that are homologous to DivIVA with a potential role in mycobacterial cell elongation. Here we describe studies on Mycobacterium smegmatis MSMEG_2416, a homologue of M. tuberculosis Rv2927c. Two previous independent studies showed that MSMEG_2416 was involved in septation (subsequently referred to as sepIVA). Contrary to these previous reports, we found sepIVA to be dispensable for growth in laboratory media by generating a viable null mutant. The mutant strain did, however, show a number of differences, including a change in colony morphology and biofilm formation that could be reversed on complementation with sepIVA as well as Rv2927c, the sepIVA homologue from M. tuberculosis . However, analysis of cell wall lipids did not reveal any alterations in lipid profiles of the mutant strain. Microscopic examination of the mutant revealed longer cells with more septa, which occurred at irregular intervals, often generating mini-compartments, a profile similar to that observed in the previous studies following conditional depletion, highlighting a role for sepIVA in mycobacterial growth.
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DOI：
10.1016/j.bbrc.2014.04.116
发表时间：
2014-06-06
影响因子：
3.1
作者：
Xu, Wen-xi;Zhang, Lu;Wang, Hong-hai
通讯作者：
Wang, Hong-hai
影响因子：
3.2
作者：
Wu, Katherine J.;Zhang, Jenna;Boutte, Cara C.
通讯作者：
Boutte, Cara C.
影响因子：
4.2
作者：
Jani C;Eoh H;Lee JJ;Hamasha K;Sahana MB;Han JS;Nyayapathy S;Lee JY;Suh JW;Lee SH;Rehse SJ;Crick DC;Kang CM
通讯作者：
Kang CM
影响因子：
10.5
作者：
Marston, AL;Thomaides, HB;Errington, J
通讯作者：
Errington, J
影响因子：
2.8
作者：
Bardarov, S;Bardarov, S;Jacobs, WR
通讯作者：
Jacobs, WR